血管内超声检测阿托伐他汀对兔动脉粥样硬化斑块的影响  

Effects of atorvastatin with intravascular ultrasound on atherosclerotic plaques in rabbits

作　　者：谭蓓[1] 关玉庆[2] 孙慧[2] 户克庆[2] 苏国海[2] 韦敏[3] 

机构地区：[1]山东大学医学院,山东济南250012 [2]山东大学附属济南市中心医院心内科,山东济南250013 [3]山东大学附属济南市中心医院供应科,山东济南250013

出　　处：《山东大学学报（医学版）》2015年第5期10-14,20,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81170087);济南市企业自主创新计划(201003112)

摘　　要：目的采用血管内超声评价短期内不同剂量阿托伐他汀对动脉粥样硬化(AS)家兔模型斑块的影响。方法 30只新西兰大白兔随机分为对照组(n=7)、模型组(n=7)、阿托伐他汀低剂量组(n=8)和阿托伐他汀高剂量组(n=8)。对照组给予普通饲料喂养,其他各组均通过8周高脂喂养联合腹主动脉球囊损伤术建立AS模型。于第8周末行血管内超声检查。8周后,模型组,阿托伐他汀低、高剂量组继续高脂喂养,分别给予低、高剂量组兔不同剂量的阿托伐他汀干预2周。10周处死所有动物。抽取兔静脉血,测定血清血脂水平,应用ELISA法测定血清超敏C反应蛋白(hs-CRP)。并行血管内超声和病理组织学检查。结果对照组血脂水平明显低于其他各组(P<0.05),阿托伐他汀低、高剂量两组血脂水平和血清hs-CRP水平明显低于模型组(P<0.05),且高剂量组变化更加明显(P<0.01)。血管内超声显示模型组的血管外弹力膜面积(EEMA)、斑块面积(PA)、管腔面积狭窄率(LAS%)明显大于低、高剂量组(P<0.05),管腔面积(LA)差异无统计学意义(P>0.05)。结论阿托伐他汀能够短时间内降低AS兔的血脂水平,并减少AS兔斑块的形成,且与药物剂量呈正相关。Objective To explore the effects of different doses of atorvastatin on atherosclerotic plaques in rabbit mod- els with intravascular ultrasound. Methods A total of 30 New Zealand white rabbits were randomized into the control group （ n = 7 ）, model group （ n = 7 ）, low-dosage atorvastain group （ n = 8 ） and high-dosage atorvastain group （ n = 8）. Except for the control group, the other groups were fed with high-fat diet for 8 weeks, and aortaventralis balloon-dilation injury was created to establish AS models. Intravascular ultrasound （ IVUS ） was performed at the end of the 8th week. After that, the model group continued to eat high-fat diet, while the two atorvastatin groups received 2-week extra atorvastatin intervention. At the end of the 10th week, all rabbits were sacrificed. Venous blood was drawn to measure serum lipid, and the amount of inflammation mediator hs-CRP was quantified with ELISA. The abdominal aortae were detected with IVUS and then observed pathologically. Results At the end of study, serum lipid in the control group was significantly lower than that in the other three groups （ P 〈 0.05 ）. Compared with model group, the level of serum lipid and hs-CRP decreased significantly in two atorvastatin groups, which were more significant in high-dosage atorvastain group （P 〈0.01 ）. Extra-elastic membranous area （EEMA）, plaque area （PA）, and lumen area stenosing （ LAS% ） in the model group were significantly higher than those in the two atorvastatin groups （ P 〈 0.05 ）, with nosignificant difference in lumen area between the model group and the two atorvastatin groups （ P 〉 0.05 ）. Conclusion Atorvastatin is able to control the level of serum lipid observably and reduce the plaque formation in atherosclerotic rabbit models.

关 键 词：血管内超声 阿托伐他汀 动脉粥样硬化 斑块 兔 

分 类 号：R541.4[医药卫生—心血管疾病] R972.6[医药卫生—内科学]