毛细管电泳法测定L-谷氨酰胺呱仑酸钠颗粒中2种主成分的含量  

Content Determination of 2 Kinds of Main Components in L-glutamine and Sodium Gualenate Granules by Capillary Electrophoresis

作　　者：周洁[1] 左利民[1] 王智亮[2] 姜威[1] 任连杰[3] 山广志[1] 

机构地区：[1]中国医学科学院医药生物技术研究所,北京100050 [2]潍坊市人民医院,山东潍坊261041 [3]北京市药品检验所,北京100035

出　　处：《中国药房》2015年第15期2106-2108,共3页China Pharmacy

基　　金：国家科技重大专项课题(No.2012ZX09301002)

摘　　要：目的：建立同时测定L-谷氨酰胺呱仑酸钠颗粒中2种主成分含量的方法。方法：采用毛细管电泳法。分离柱为未涂层熔融石英毛细管柱,运行缓冲液为40 mmol/L硼砂缓冲液（p H=9.22）,样品缓冲液为4 mmol/L硼酸缓冲液（p H=6.97）,温度为20℃;进样方式为压力进样,进样压力为0.5 psi,进样时间为5 s,分离电压为15 kV,检测波长为220 nm。结果：L-谷氨酰胺和呱仑酸钠质量浓度分别在0.332 5-~13.30 mg/ml（r=0.999 3）和1.00-40.0μg/ml（r=0.998 3）范围内与各自峰面积呈良好的线性关系;精密度、稳定性试验的RSD≤2.83%;平均加样回收率分别为98.33%（RSD=4.50%,n=9）和100.03%（RSD=4.98%,n=9）。结论：该方法操作简便,结果灵敏、准确性高,可用于L-谷氨酰胺呱仑酸钠颗粒中2种主成分的含量测定。OBJECTIVE：To establish a method for the content determination of 2 kinds of main components in L-glutamine and sodium gualenate granules.METHODS：Capillary electrophoresis was conducted.The separation column was uncoated silica capillary column with the running buffer of 40 mmol/L borax buffer solution（p H=9.22）and the sample buffer of 4 mmol/L boric acid buffer solution（p H=6.97）;the temperature was 20 ℃;the injection pressure was 0.5 psi and injection time was 5 sec;the separation voltage was 15 kV and detection wavelength was 220 nm.RESULTS：There was a good linear relationship between the quality concentration of L-glutamine in the range of 0.332 5-13.30 mg/ml（r=0.999 3）and sodium gualenate was in the range of 1.00-40.0 μg/ml（r=0.998 3）.The RSDs of precision and stability test were no more than 2.83%;the average recovery was respectively 98.33%（RSD=4.50%,n=9）and 100.03%（RSD=4.98%,n=9）.CONCLUSIONS：The method is simple,sensitive,accurate and can be used for the content determination of 2 kinds of main components in L-glutamine and sodium gualenate granules.

关 键 词：毛细管电泳 L-谷氨酰胺呱仑酸钠颗粒 L-谷氨酰胺 呱仑酸钠 含量测定 

分 类 号：R917[医药卫生—药物分析学]