ELISA法检测丙型肝炎病毒抗体最佳临界值及其可疑区间  

The determination of optimal cut-off value and its dubious interval for detection of anti-HCV antibody by ELISA

作　　者：卢香云[1] 程江[1] 包建玲[2] 张朝霞[2] 张丽翠[3] 曹文疆[3] 文艺竹 

机构地区：[1]新疆石河子大学医学院,新疆石河子832000 [2]新疆医科大学第一附属医院,新疆乌鲁木齐830000 [3]新疆石河子大学医学院第一附属医院检验科,新疆石河子832000

出　　处：《现代预防医学》2015年第10期1841-1844,共4页Modern Preventive Medicine

基　　金：卫生部医药卫生科技发展研究中心专项课题(28-1-13)

摘　　要：目的探讨ELISA法检测丙型肝炎病毒（HCV）抗体诊断阳性的最佳临界值（Cut-off值）及其可疑区间,为不同实验室间的结果互认提供依据。方法选取某院住院或体检的130例经ELISA法初筛为阳性的血清标本和25例接近临界值（阴性）标本（OD=0.15～2.0）,采用重组免疫印记（RIBA）法检测确认其阴、阳性。利用SPSS17.0绘制ROC曲线,确定最佳Cut-off值,进一步探讨可疑区间。结果 ELISA法检测HCV抗体诊断阳性的最佳Cut-off值为0.2513,其可疑区间范围为0.2136～0.5513。结论确定适合本实验室最佳Cut-off值和可疑区间能有效地降低丙型肝炎抗体检测的漏诊率和误诊率。Objective The study assessed the optimal cut-off value and its dubious interval of ELISA in detection of anti-HCV antibody, aiming to provide a reference for the mutual reliability of test results from different laboratories. Methods 130 serum specimens tested positive of anti-HCV antibody in a preliminary ELISA screening from hospitalized patients or physical examinees at our hospital were selected; meanwhile, 25 specimens tested negative or close to the cut-off value（OD=0.15-2.0） were also selected. Recombinant Western Blot（RIBA） was used to confirm their positivity and negativity. An ROC curve was prepared by using SPSS17.0 to identify the optimal cut-off value and explore the dubious interval. Results The optimal cut-off value of ELISA in detecting anti-HCV antibody was 0.2513, with a dubious interval ranging from 0.2136 to 0.5513. Conclusion Confirming the optimal cut-off value and dubious interval suitable for our laboratory can effectively help reduce the incidences of missed diagnosis and misdiagnosis in detection of anti-HCV antibody.

关 键 词：丙型肝炎抗体 ROC曲线 临界值 可疑区间 

分 类 号：R446[医药卫生—诊断学]