反相高效液相色谱法测定去甲斑蝥素齐墩果酸复合脂质体包封率  

RP-HPLC Determination of Entrapment Efficiency of Norcantharidin and Oleanolic Acid Complex Liposome

作　　者：方志娥[1] 陈薇[1] 唐直婕[2] 冷静[1] 

机构地区：[1]重庆市中医院,重庆400021 [2]重庆医科大学药学院,重庆400016

出　　处：《中国药业》2015年第10期26-28,共3页China Pharmaceuticals

基　　金：重庆市自然科学基金项目;项目编号:CSTC;2010BB5347;重庆市卫生局中医药科技项目;项目编号:2011-2-161

摘　　要：目的建立测定去甲斑蝥素和齐墩果酸复合脂质体包封率的反相高效液相色谱（RP-HPLC）法。方法色谱柱为Thermo Sycronis C18柱（150 mm×4.6 mm,5μm）,梯度洗脱,检测波长为210 nm,柱温为30℃。超速离心法分离脂质体中游离药物。结果去甲斑蝥素质量浓度在20-300μg/m L范围内与峰面积线性关系良好,r=0.999 8,平均回收率为99.15%-100.11%,RSD≤3.20%（n=5）;齐墩果酸质量浓度在10-150μg/m L范围内与峰面积线性关系良好,r=0.999 7,平均回收率为99.22%-100.30%,RSD≤3.34%（n=5）。结论该方法简便、快速,可用于去甲斑蝥素齐墩果酸复合脂质体包封率的测定。Objective To establish a reversed-phase high performance liquid chromatography（RP-HPLC） method for the determination of entrapment efficiency of norcantharidin and oleanolic acid complex liposome. Methods The separation was achieved by using the Thermo Sycronis C18 column （ 150 mm × 4. 6 mm,5 μm ） with the gradient elution. The detection wavelength was set at 210 nm and the column temperature was 30 ℃. The free drugs in liposome were separated by using the upercentrifugation. Results The mass concentra-tion of norcantharidin in the range of 20-300 μg/mL showed the good linear relation with the peak area and the average recovery was 99. 15% -100. 11%,RSD ≤ 3. 20%（ n=5）;the mass concentration of oleanolic acid in the range of 10-150 μg/mL showed the good linear relation with the peak area and the average recovery was 99. 22% -100. 30%,RSD≤3. 34%（ n=5）. Conclusion The method is simple,fast,and thus can be applied to the determination of entrapment efficiency of norcantharidin and oleanolic acid com-plex liposome.

关 键 词：反相高效液相色谱法 脂质体 去甲斑蝥素 齐墩果酸 

分 类 号：TQ461[化学工程—制药化工] R979.1[医药卫生—药品]