高效液相色谱法测定四神片中吴茱萸碱和吴茱萸次碱含量  

Determination of Evodiamine and Rutaecarpine Contents in Sishen Tablets by HPLC

作　　者：潘瑞玲[1] 韩慧琴 

机构地区：[1]内蒙古医学院第二附属医院,内蒙古呼和浩特010030 [2]河北省张家口市食品药品检验中心,河北张家口075000

出　　处：《中国药业》2015年第10期60-62,共3页China Pharmaceuticals

摘　　要：目的建立测定四神片中吴茱萸碱和吴茱萸次碱含量的高效液相色谱（HPLC）法。方法使用Waters 2695-2996型高效液相色谱仪,色谱柱为Capcell PAK-C18柱（150 mm×4.6 mm,5μm）,流动相为0.1%磷酸溶液（含十二烷基硫酸钠0.2%）-甲醇-乙腈（50∶20∶30）,吴茱萸碱、吴茱萸次碱检测波长分别为225 nm和343 nm,流速为1.0 m L/min。结果吴茱萸碱的进样量线性范围为0.003 8-0.750 0μg（r=0.999 9）,平均回收率为100.19%,RSD为0.18%（n=6）;吴茱萸次碱的进样量线性范围为0.002 5-0.500 0μg（r=0.999 9）,平均回收率为100.10%,RSD为0.44%（n=6）。结论该方法简便、准确、重复性好,可用于测定四神片中吴茱萸碱和吴茱萸次碱的含量。Objective To establish a HPLC method for determining the evodiamine and rutaecarpine contents in Sishen Tablets. Methods The Waters 2695-2996 type HPLC was adopted with Capcell PAK-C18 column（150 mm × 4. 6 mm,5 μm）as fixed phase and 0. 1%phosphoric acid solution（containing 0. 2% sodium dodecylsulfate）-methanol-acetonitrile（50 ：20 ：30）as the mobile phase. The detec-tive wave length was 225 nm for evodiamine and 343 nm for rutaecarpine. The flow rate was 1. 0 mL/min. Results The linearity range of evodiamine was 0. 003 8-0. 750 0 μg（ r=0. 999 9）,the average recovery rate was 100. 19%,RSD=0. 18%（ n=6）;the lin-earity range of rutaecarpine was 0. 002 5-0. 500 0 μg（ r=0. 999 9）,the average recovery rate was 100. 10%,RSD=0. 44%（ n=6）. Conclusion The method is simple,accurate,better reproducible and suitable for the quantitative determination of evodiamine and rutae-carpine contents in Sishen Tablets.

关 键 词：四神片 吴茱萸碱 吴茱萸次碱 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]