阿托伐他汀对高糖诱导的系膜细胞凋亡的影响  

Effect of atorvastatin on apoptosis of hyperglycemia induced mesangial cells in rat

作　　者：朱丹[1] 胡莹[1] 赵欣[2] 马吉芳[1] 郝建兵[1] 任连生[1] 崔蕾[1] 

机构地区：[1]哈尔滨医科大学附属第一医院肾内二病房,150070 [2]黑龙江省医院血液透析中心,哈尔滨150001

出　　处：《检验医学与临床》2015年第9期1196-1197,共2页Laboratory Medicine and Clinic

基　　金：黑龙江省教育厅科学技术研究项目(12521293)

摘　　要：目的探讨不同浓度阿托伐他汀对大鼠系膜细胞凋亡的影响。方法体外培养大鼠永生系膜细胞,分为5组:对照(C)组;高糖(30mmol/L)(H)组;高糖(30mmol/L)+阿托伐他汀(10-7 mmol/L)(H+A1)组;高糖(30mmol/L)+阿托伐他汀(10-6 mmol/L)(H+A2)组;高糖(30mmol/L)+阿托伐他汀(10-5 mmol/L)(H+A3)组。采用Annexin V-FITC和PI双染法检测细胞凋亡。采用caspase-3活性检测试剂盒检测系膜细胞caspase-3活性。结果与C组相比,H组系膜细胞凋亡率明显增加,caspase-3活性明显增加,差异有统计学意义(P<0.05)。与H组相比,高糖加不同浓度阿托伐他汀组细胞凋亡率明显降低,caspase-3活性明显降低,差异有统计学意义(P<0.05),且呈浓度依赖。结论阿托伐他汀可以通过caspase途径抑制醛固酮诱导的大鼠系膜细胞凋亡。Objective To investigate the influence of different concentrations of atorvastatin on apoptosis of rat mesangial cells .Methods Rat immortal mesangial cells were cultured in vitro and divided into 5 groups：control （C） group ,hyperglycemia （30 mmol/L ,H）group ,hyperglycemia （30 mmol/L）＋ atorvastatin（10^-7 mmol/L）（H＋A1） group ,hyperglycemia （30 mmol/L）＋ atorvastatin（10^-6 mmol/L）（H ＋ A2）group and hyperglycemia （30 mmol/L）＋atorvastatin（10^-5 mmol/L）（H＋ A3）group .Apoptosis was detected by Annexin V‐FITC and PI double staining ,and the caspase‐3 activity was assayed by the caspase‐3 activity kit .Results Compared with the group C , the mesangial cells apoptosis rate and caspase‐3 activity in the group H were significantly increased ,the differences were statistically significant（P〈0 .05） .Compared with the group H ,the mesangial cells apoptosis rate and caspase‐3 activity in the H＋ A1 ,2 ,3 groups were significantly decreased ,the differences were statistically significant （ P〈0 .05） ,moreover which showing the concentration dependence .Conclusion Atorvastatin may inhibit aldosterone in‐duced mesangial cells apoptosis via caspase pathway .

关 键 词：阿托伐他汀 高糖 系膜细胞 凋亡 CASPASE-3 

分 类 号：R692.602[医药卫生—泌尿科学]