粤西地区5种荔枝nrDNA ITS基因扩增及酶切分析研究  

Amplification and Enzyme Digestion Analysis of nrDNA ITS Gene in 5 Kinds of Litchi Cultivars from the Western Part of Guangdong Province

作　　者：黎翠萍 陈玉莹 袁长春 黎海利 刘锴栋 

机构地区：[1]岭南师范学院生命科学与技术学院,广东湛江524048

出　　处：《现代农业科技》2015年第9期64-67,共4页Modern Agricultural Science and Technology

基　　金：国家级大学生创新创业训练计划项目(201310579010);国家星火计划项目(2013GA780093;2013GA780080);广东省科技计划项目(2013B020304008);广东省教育厅科技创新项目(2013KJCX0124);湛江科技攻关计划项目(2011C3106019;2012C3102019);湛江师范学院科研创新团队资助项目(2013CXTD05);湛江市热带特色资源植物技术开发重点实验室项目(2014A06008)

摘　　要：对广东省粤西地区的5个荔枝品种进行核糖体DNA（rDNA）内转录间隔区（ITS）的PCR扩增,并用限制酶HhaI、HaeⅢ、EcoRⅠ、HindⅢ、RsaⅠ对扩增产物进行酶切分析。5种荔枝属植物的ITS基因序列长度为600-800bp,PCR产物经过5种酶切后,5种荔枝属植物的PCR产物均切出位于600-800bp之间的主带,妃子笑的主带与其余4种差异明显;结果表明,荔枝品种中的白糖罂、新兴、糯米糍、尚书怀4种的亲缘性比较近,妃子笑与这4种的亲缘性较远。The PCR amplifications of ITS in Ribosomal DNA（rDNA）of five kinds of litchis grown in Guangdong Province were researched,using restriction enzyme HhaI,HaeⅢ,EcoR Ⅰ,HindⅢ,RsaⅠinto the enzyme digestion analysis.All these five kinds of litchis’ ITS genetic sequence lengths were 600-800 bp.After the enzyme digestion of PCR amplification,five kinds of litchis’ PCR amplifications were digested in the position of main belt between 600-800 bp.The litchis of Feizixiao were different from all other four spices in the main belt.The research results also revealed that the litchis of Baitangying,Xinxing,Nuomici and Shangshuhuai were relatively closed in the aspect of affinity,while the litchis of Feizixiao were more distant.

关 键 词：荔枝 ITS基因 酶切分析 基因扩增 粤西地区 

分 类 号：S667[农业科学—果树学]