PB和TPM对未成熟脑大鼠远期学习记忆的影响及刺五加的保护作用  

Influence of Phenobarbital and Topiramate on long-term learning and memory in Rats with immature brain and the protection effect of Acanthopanax

作　　者：葛利吉[1] 杨琳[2] 韩玲[1] 刘宇[2] 齐薛浩[3] 

机构地区：[1]陕西中医学院,陕西咸阳712000 [2]西安交通大学医学院第二附属医院,陕西西安710000 [3]西安市儿童医院,陕西西安710000

出　　处：《中国妇幼健康研究》2015年第2期207-209,245,共4页Chinese Journal of Woman and Child Health Research

基　　金：国家自然科学基金资助项目(30973224);医院人才培养专项科研基金资助项目[RC(XM)200908];陕西省科技公关资助项目[2006k15-G1(2)]

摘　　要：目的通过短期给予新生幼鼠不同抗癫痫药,观察苯巴比妥(PB)和托吡酯(TPM)对幼鼠远期学习记忆功能的影响及刺五加的保护作用。方法实验动物分两批:第1批3窝健康3日龄Sprague-Dawley(SD)大鼠30只(雌、雄不分)随机分为3组,生理盐水对照组10只、苯巴比妥组10只、苯巴比妥+刺五加组10只;第2批3窝健康3日龄Sprague-Dawley(SD)大鼠30只(雌、雄不分)随机分为3组,生理盐水对照组10只,托吡酯组10只,托吡酯+刺五加组10只。对照组给予生理盐水10mL·kg^(-1)·d^(-1),苯巴比妥组:苯巴比妥62.5mg·kg^(-1)·d^(-1),苯巴比妥+刺五加组:苯巴比妥62.5mg·kg^(-1)·d^(-1),刺五加干粉5.6g·kg^(-1)·d^(-1)。托吡酯组:40mg·kg^(-1)·d^(-1),托吡酯+刺五加组:托吡酯40mg·kg^(-1)·d^(-1),刺五加干粉5.6g·kg^(-1)·d^(-1)。每日称重后腹腔注射,连续3天。正常饲养至1月龄,最后各组选取8只大鼠,参加水迷宫测试。结果苯巴比妥干预组大鼠寻找水下平台的潜伏期较对照组明显延长,均存在显著性差异(t=-3.542,P=0.005);穿越有效区次数较对照组减少(t=3.352,P=0.005)。加用刺五加保护后,与对照组比较,潜伏期(t=-1.313,P=0.210)及穿越有效区次数(t=0.051,P=0.128)均无显著性差异。托吡酯干预组大鼠与对照组比较潜伏期(t=-1.920,P=0.075)、穿越有效区次数(t=1.915,P=0.076);托吡酯组与托吡酯+刺五加组比较潜伏期(t=-0.597,P=0.560)、穿越有效区次数(t=1.660,P=0.119),均无显著性差异。结论未成熟脑短期应用苯巴比妥损害远期学习记忆,刺五加具有保护作用,短期应用中等剂量的托吡酯不会对未成熟脑产生远期学习记忆障碍。Objective To observe the influence of Phenobarbital （ PB） and Topiramate （ TPM） on long-term learning and memory of newly-born rats by short-term administration and the protection effect of Acanthopanax.Methods There were two batches of rats.Among the first batch, 30 3-day old healthy Sprague-Dawley （ SD） rats were randomly divided into 3 groups, control group, PB group and PB combined with Acanthopanax group.Among the second batch, 30 3-day old healthy Sprague-Dawley （ SD） rats were randomly divided into 3 groups with 10 rats in each, control group, TPM group and TPM combined with acanthopanx group.Hypertonic saline （10mL· kg^-1· d^-1 ） was given to the control group, PB group was injected with PB （62.5mg· kg^-1· d^-1）, and PB （62.5mg· kg^-1· d^-1） and Acanthopanax （5.6g· kg^-1· d^-1 ） were injected to PB combined with Acanthopanax group.TMP was injected to TMP group with the dose of 62.5mg·kg^-1· d^-1 , and TMP combined with Acanthopanax group were given 62.5mg· kg^-1· d^-1 of TMP and 5.6g· kg^-1· d^-1 of Acanthopanax.The rats were injected after weighed for three continuous days.They were regularly fed till they were one month old.Finally 8 out of each group were selected to attend the game of Morris water maze.Results The incubation period for searching underwater platform in PB group was longer than the control group, and the difference was significant （ t=-3.542,P=0.005） .The times passing through the effective area reduced in PB group compared with the control group （t=3.352,P=0.005）.However, after adding Acanthopanax, the incubation period （t=1.313,P=0.210） and the times passing through the effective area （t =0.051,P=0.128） had no significant difference from the control group.The incubation period for searching underwater platform in TPM group was significantly longer than the control group （t=-1.920,P=0.075）, but the times passing through the effective area was less than the control group （t=1.915,P=0.076） .However, TPM group was not s

关 键 词：苯巴比妥 托吡酯 刺五加 未成熟脑 学习记忆 

分 类 号：R720.597[医药卫生—急诊医学]