肝X受体激动剂诱导获得的小鼠耐受性树突状细胞与天然耐受性树突状细胞的比较  

Comparison between mouse tolerant dendritic cells induced by liver X receptor agonist and natural tolerant dendritic cells

作　　者：徐海燕[1] 马旭怡[1] 董盼盼[1] 薛冬[1] 何小舟[1] 

机构地区：[1]苏州大学附属第三医院泌尿外科,常州213003

出　　处：《国际免疫学杂志》2015年第3期208-213,共6页International Journal of Immunology

基　　金：江苏省常州市卫生局重大项目（ZD201111）

摘　　要：目的 通过与天然耐受性树突状细胞（N-tDCs）比较来探讨肝X受体（LXR）激动剂诱导获得的小鼠耐受性树突状细胞T-tDCs的免疫耐受特性.方法 获取小鼠骨髓来源单个核细胞,以白细胞介素（IL）-4和粒细胞-巨噬细胞集落刺激因子（GM-CSF）辅以LXR激动剂（T0901317）诱导7d后,检测细胞表型、进行混合淋巴细胞反应、调节性T细胞（Tregs）体外诱导实验等;或继续以脂多糖（LPS）诱导其成熟,进行表型分析.利用BALB/c DO11.10 RAG-/-小鼠来源的CD4＋T细胞进行Tregs诱导实验,并与磁珠阴性分选从小鼠脾脏获得CD4＋ DCs （N-tDCs）进行对照研究.结果 与N-tDCs比较,T0901317诱导7d时的T-tDCs形态更接近普通DCs,CD80、CD86、MHC-Ⅱ类分子表达略低,但均为中低表达,CCR7表达水平相近.N-tDCs具有更强的抑制能力,当特异性抗原鸡卵清白蛋白（OVA）存在时,N-tDCs可以更强地诱导Tregs的生成,而T-tDCs可以分泌更高水平的IL-10.结论 T-tDCs与N-tDCs以不同的方式发挥免疫抑制作用.Objective To explore the characteristics of tolerant dendritic cells （tDCs） induced by liver X receptor （LXR） agonist by comparing with natural tDCs （N-DCs）.Methods Mononuclear cells from mouse bone marrow were stimulated by interleukin （IL）-4 and granulocyte-macrophage colony stimulating factor （GM-CSF） in the presence or absence of the stimulation by LXR agonist-T0901317 for 7 days,or induced by lipopolysaccharide （LPS） for 3 days.Then phenotype detection,mixed lymphocyte reaction （MLR）,and regulatory T cells （Tregs） induction assay were performed.CD4 ＋ T cells purified from BALB/c DO11.10 RAG-/-mouse were used in Tregs induction assay.N-tDCs isolated from mouse spleen were used as controls.Results Compared with N-tDCs,the morphology of T-tDCs induced by T0901317 for 7 days were similar to normal DCs＇,but with little lower or moderate expression levels of CD80,CD86 and MHC-Ⅱ.Stronger inhibitory effect of N-tDCs was observed,and their Tregs induction ability was enhanced in the presence of ovalbuamin （OVA）.T-tDCs can secret higher level of IL-10.Conclusion T-tDCs and N-tDCs may play the inhibitory role through different mechanisms.

关 键 词：耐受性树突状细胞 肝X受体 调节性T细胞 白细胞介素4 

分 类 号：R392[医药卫生—免疫学]