用CODEHOP法设计简并引物克隆草鱼多聚免疫球蛋白受体基因片段  

Cloning of polymeric immunoglobulin receptor gene fragment in grass carp Ctenopharyngodon idellus using degenerate primers designed by CODEHOP

作　　者：许国晶 王超 孟庆磊 刘峰 刘飞 陈秀丽 

机构地区：[1]山东省淡水渔业研究院山东省盐碱地渔业工程技术研究中心,济南250013

出　　处：《大连海洋大学学报》2015年第2期138-142,共5页Journal of Dalian Ocean University

基　　金：国家自然科学基金资助项目(31440090);山东省农业良种工程项目;山东省财政支持农业技术推广项目

摘　　要：为克隆草鱼Ctenopharyngodon idellus多聚免疫球蛋白受体p IgR基因,本研究中借助于NCBI、Blast等数据库及Primer Premier 5.0、DNAMAN等生物软件,利用CODEHOP法设计了简并引物,并对克隆基因进行同源性比较和系统发生分析。结果表明:用CODEHOP法设计的简并引物特异性强,试验成功率高;草鱼p IgR基因属于整个脊椎动物的p IgR基因群,与鲤Cyprinus carpio同源性最高,为84%。The gene of polymeric immunoglobulin receptor （pIgR）of grass carp Ctenopharyngodon idellus was cloned by a pair of degenerate primers designed by CODEHOP, and by combined bioinfomnatics including NCBI, Blast and biological software such as Primer Premier 5.0 and DNAMAN, and the homology and phylogenetic tree of the clone ofplgR gene were analyzed in the PCR products. The results showed that degenerate primers designed by CODEHOP were characterized by specificity and benefit for the experiment accomplishing, plgR gene of grass carp was found to clustered into plgR gene group in all the other vertebrates, especially into the fish subgroup, with the maximal homology （84%） with common carp Cyprinus carpio.

关 键 词：草鱼 CODEHOP 多聚免疫球蛋白受体(p IgR) 

分 类 号：S965.111[农业科学—水产养殖] Q512.2[农业科学—水产科学]