Screening and Isolation of Highly Efficient Cellulose-degrading Microorganisms and Construction of Complex Microbial System  

高效降解纤维素菌系的筛选分离及复合菌系的构建（英文）

作　　者：尚婷婷[1] 李全宏[2] 邓尚贵[1] 

机构地区：[1]浙江海洋学院食品与医药学院,浙江舟山316022 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《Agricultural Science & Technology》2015年第4期639-644,652,共7页农业科学与技术（英文版）

基　　金：Supported by Key Program for International S&T Cooperation Projects of China(2012DFA30600);National Key Technology Research and Development Program(2012BAD29B06);Special Scientific Research Fund of Marine Public Welfare Profession of China(201305013)~~

摘　　要：This study almed to screen a highIy efficient ceI uIose-degrading compIex microbial system from soiI and rotten straw and then study its appIication to natural ceI uIose. The isoIated stralns were preIiminariIy screened with Congo red stalning and the ceI uIases activities were determined with DNS method. The non-antagonis-tic highIy efficient ceI uIos-degrading stralns were seIected and cuItured combinedIy for deveIoping a ceI uIose-degrading compIex microbial system. The resuIts showed that the CMC enzyme activity of the mixed cuIture of the three fungi stralns was higher than those of the cuItures of the three singIe stralns. The morphoIogical and moIecuIar bioIogical identification indicated that F1 was Botryosphaeria, F2 was Rhi-zopus oryzae, and F5 was Fusarium oxysporum. When straw was used as carbon source, the CMC enzyme activities of F1, F2 and F5 were 39.2, 31.4 and 40.6 IU/mI, respectiveIy; whiIe the CMC enzyme activity of the mixed cuIture of F1＋F2＋F5 was 50.12 IU/mI, which was increased by 23% compared to that of the singIe straln of F5. The experimental resuIts indicated that the ceI uIose-degrading effect of the compIex microbial system was better than those of the singIe fungi stralns, and the singIe stralns of F1, F2 and F5 had certaln deveIopment potentials.为了从土壤及腐烂的秸秆中筛选一组高效降解纤维素的复合菌系,并研究其在天然纤维素中的应用。通过采用刚果红染色液法对分离的菌株初步筛选,利用DNS法测定纤维素酶活力。选取无拮抗高效降解纤维素菌株进行组合培养构建降解纤维素复合菌系。结果表明,3株真菌混合培养后酶活力效果优于单一菌株。经过形态学和分子生物学鉴定,真菌F1为葡萄座腔菌(Botryosphaeria)、F2为米根霉(Rhizopus oryzae)及F5为尖孢镰刀菌(Fusarium oxysporum)。复合培养后,在碳源为秸秆时,单菌株酶活值分别为F1 39.2 IU/ml,F2 31.4 IU/ml,F5 40.6 IU/ml,真菌组合F1+F2+F5培养后酶活值为50.12 IU/ml,复合真菌系酶活值比F5单菌株提高了23%。通过实验研究得出复合菌系对纤维素的降解效果优于单一菌株,菌株F1、F2和F5具有潜在的开发价值。

关 键 词：CeI ulose degradatlon Screening and isolatlon Identificatlon 

分 类 号：Q93[生物学—微生物学]