Effects of Bacillus amyloliquefaciens Biofertilizer on Brassica juncea var. multiceps Growth and N_2O Emission from Soil  

解淀粉芽孢杆菌菌剂对雪菜生长和土壤氧化亚氮排放的影响（英文）

作　　者：白志辉[1] 王璠[1] 曹建喜[2] 吴尚华[1] 徐圣君[1] 马双龙[1] 

机构地区：[1]中国科学院生态环境研究中心,北京100085 [2]唐山市环境保护局丰润分局,河北唐山064000

出　　处：《Agricultural Science & Technology》2015年第4期727-732,749,共7页农业科学与技术（英文版）

基　　金：Supported by Key Research Program of the Chinese Academy of Sciences(KZZD-EW-09-3,KZZD-EW-11-03);National Science and Technology Major Project(2014ZX07204-005);Special Fund of the National Academy Alliance(2012-1)~~

摘　　要：In this study, Bacil us amyloliquefaciens A3 was continual y incubated in shake fIasks contalning wastewater from sweet potato starch production as an ef-fective biofertiIizer for cuItivation of Brassica juncea var. multiceps（XueIihong）. Based on pot experiments in the greenhouse, the effects of chemical fertiIizers （CN）, biofertiIizer （BF）, inactivated broth （BI）, starch wastewater （SW） and the combination of biofertiIizer and chemical fertiIizer （BC） on the yield, NO3- content and NO2- con-tent of XueIihong, soiI physicochemical properties and N2O emission were investi-gated. The resuIts showed that the yield of XueIihong in BC and CN treatments was improved by five times compared with CK; BF and SW treatments had insignifi-cant impact on the yield of XueIihong. Compared with CN treatment, BCL treatment exhibited simiIar improving effects on the yield of XueIihong, in which NO3- content of XueIihong and soiI was reduced by 16.4%-73.6% and 22%-29%, which reduced the risk of nitrogen eIuviations in soiI; average N2O fIux （FPV30） in BCL treatment was reduced by 58.3%-73.1% compared with CN treatment. In concIusion, B. amy-loliquefaciens is a feasibIe Iow-cost biofertiIizer for sustalnabIe vegetabIe farming with a great potential for starch wastewater utiIization.由于化肥的过度使用对环境的不利影响，微生物肥料的研究得到了广泛关注。实验利用甘薯淀粉废水培养解淀粉芽孢杆菌并将其作为微生物肥料用于雪菜种植。通过盆栽实验考察了尿素（CN）、解淀粉芽孢杆菌菌液（BF）、解淀粉芽孢杆菌灭活菌液（BI）、甘薯淀粉废水（SW）、尿素结合微生物肥料（BC）对蔬菜产量、NO3-、NO2-含量，以及土壤性状， N2O排放的影响。试验表明：BC和 CN在蔬菜产量方面比CK提高了5倍。BF和 SW对蔬菜产量影响不显著。半量氮肥配合菌肥处理（BCL）表现出与 CN相近的增产效果，而蔬菜 NO3-含量同 CN处理相比下降了16.4%~73.6%，土壤NO3-含量降低了22%~29%，降低了土壤中氮淋溶风险；土壤 N2O（FPV30）平均排放量较 CN处理降低了58.3%~73.1%。综上，利用淀粉废水培养解淀粉芽孢杆菌不仅可以资源化利用废水，并且其产物可作为一种绿色肥料应用于蔬菜种植，为淀粉废水资源化利用和农田温室气体减排提供了一个新思路。

关 键 词：Bacil us amyloliquefaclens Wastewater from sweet potato starch pro-ductlon Brassica juncea var. multiceps Blofertliizer N2O emisslon 

分 类 号：S637.2[农业科学—蔬菜学]