浙江省55例HIV抗体检测不确定结果的随访复检与处理对策  

Follow-up testing and resolution strategy of 55 cases of indeterminate HIV antibody in Zhejiang province

作　　者：夏燕[1] 郭志宏[1] 张佳峰[1] 丁晓贝[1] 黄晶晶[1] 张文君[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310051

出　　处：《中国艾滋病性病》2015年第4期283-286,共4页Chinese Journal of Aids & STD

摘　　要：目的分析艾滋病病毒（HIV）抗体检测产生不确定结果的特点，探索HIV抗体不确定的处理对策。方法经HIV抗体蛋白免疫印迹试验（WB）检测结果不确定者作为研究对象，进行随访检测并对结果和相关信息进行分析。结果2009-2013年，浙江省经WB检测HIV抗体不确定者共计55例，经随访复查，34例确证阳性（61．8％），18例确证阴性（32．7％），3例不确定／失访（5．5％），共有13种带型组合模式。52．9％的研究对象2周随访发生阳转。酶联免疫吸附实验（ELISA）S／CO值〉6．0组阳转率高于S／CO值0～6．0组（χ^2=11．898，P〈0．01）。所有核酸检测阳性样品（34例）最后均发生阳转，所有核酸检测阴性样品（16例）最后均未发生阳转（其中1例失访）。结论WB不确定结果与检测对象的临床状态有关，根据各检测结果的特点，提前或延长随访复检时间、核酸检测和综合流行病学资料分析，有助于不确定结果的解释和对艾滋病的正确诊断。Objective To analyze the characteristics of indeterminate results by HIV antibodies test and explore resolution strategy of indeterminate HIV antibody results. Methods 55 cases of HIV ontibadies uncertainuy were caunched follow-up testing. We analyzed the results and related information. Results Among fifty five cases of fol- low-up testing, 34 cases were confirmed as positive （61.8%）, 18 cases were confirmed as negative （32.7%）, 3 cases were not sure/lost to follow-up （5.5%）, including a total of 13 types of model. 52.9% indeterminate cases turned positive in two weeks of follow-up. Rate of HIV seroconversion by ELISA test in S/CO value〉 6.0 group was higher than in the S/CO value 0-6.0 group （ Z2 = 11. 898, P〈0.01）. A total of 34 cases with HIV serocon- version were confirmed as HIV positive by nucleic acid testing. A total of 16 cases were confirmed as sero-negative by nucleic acid testing, no one was in HIV seroconversion. Conclusion The different HIV/AIDS clinical periods or a certain period of the general population may have indeterminate results of HIV antibody detection. Based on the characteristics of the different test method, early and delayed follow-up and combined with nucleicacid detection and comprehensive; judgment of epidemiological investigation would be conducive to the interpretation of HIV antibodies uncertainly, and correct diagnosis of AIDS.

关 键 词：艾滋病病毒抗体 蛋白免疫印迹试验 不确定结果 核酸检测 

分 类 号：R373.9[医药卫生—病原生物学]