利用F_2群体定位水稻谷粒长宽比QTL  

QTLs Mapping of Grain Length-width Ration by Using F_2 Population in Rice

作　　者：马孟莉[1] 刘艳红[1] 江玲[2] 雷恩[1] 苏一兰[1] 卢丙越 

机构地区：[1]红河学院生命科学与技术学院/云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661100 [2]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《江西农业大学学报》2015年第2期260-263,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：云南省应用基础研究计划项目(2013FZ124);云南省教育厅科学研究基金项目(2013Y066);红河学院中青年学术骨干培养计划(2014GG0101);红河学院博士科研启动专项项目(14bs17)

摘　　要：以粳稻南粳35和籼稻N22杂交得到的F2群体为研究对象,通过构建遗传连锁图谱,对控制水稻谷粒长宽比的QTL进行定位。谷粒长宽比在F2群体中呈近似的正态分布,表现为数量性状特征;利用Win QTLcart2.5软件共检测到3个控制谷粒长宽比的QTL,分别位于第3、4和5染色体上,单个QTL对表型的贡献率介于4.97%-58.06%,加性效应值介于0.05-0.20,除q LWR-4外,谷粒长宽比增效基因均来自长粒型水稻品种N22,其中q LWR-5对谷粒长宽比表型贡献率最大;基因作用方式表现为超显性、显性和部分显性;此外,利用QTLNetwork2.0软件没有检测到上位性QTL。Taking the F2 population derived from a cross between Nanjing35 and N22 as the research object, the QTLs controlling grain length-width ratio were mapped through the construction of a genetic linkage map.In the F2 population,approximate normal distribution indicated the quantitative characters of grain length- width ratio.Three putative QTLs were identified on chromosomes 3,4,5 using Win QTLcart 2.5.The percentage of phenotypic variance explained （PVE） ranged from 4.97% to 58.06% with the additive effect of a single QTL ranging from 0.05 to 0.20.Except for qLWR-4, alleles of these QTLs were from length-grain variety N22. qLWR-5 had the major effect on grain length-width ratio.The modes of gene action were mainly overdominance, dominance and oartial dominance. No epistatic QTLs were detected in the study.

关 键 词：水稻 谷粒长宽比 QTL定位 

分 类 号：S511.032[农业科学—作物学]