玉米交换单株高效筛选体系的研究  

Study on improved system of screening recombinants for fine mapping in maize

作　　者：温晶晶[1] 高景阳 王永霞[2] 赵荣兵[2] 丁俊强[2] 吴建宇[1] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002 [2]河南农业大学农学院,河南郑州450002

出　　处：《河南农业大学学报》2015年第2期135-139,共5页Journal of Henan Agricultural University

基　　金：河南农业大学农学院青年科学基金项目(30800688)

摘　　要：本研究用改良碱煮法提取玉米叶片基因组DNA,探索了DNA模板量、引物量、PCR保护剂对PCR反应的影响。结果表明,当DNA模板原液稀释5倍,质量浓度为27.3 ng·μL-1,且反应体系中加入保护剂PVP-40和BSA时可有效提高PCR效率,结合双重PCR的方法实现了快速高效的交换单株筛选。In this study,a modified alkaline lysis method was used for the genomic DNA extraction from maize leaf,and then the amount of DNA template was studied and the PCR protectants,PVP-40 and BAS,were used. The results show that it was efficient for PCR reaction when DNA template was27. 3 ng·μL^-1 and the protectants were used. In addition,the double PCR with the two sets of primers can be used to screen the recombinants with high efficiency.

关 键 词：玉米 DNA提取 碱煮法 交换单株 PCR保护剂 双重PCR 

分 类 号：S513[农业科学—作物学]