3种金黄色葡萄球菌基因分型技术的评估  

Evaluation on three detection methods for genotyping of Staphylococcus aureus

作　　者：王冰[1] 李迎慧[1] 

机构地区：[1]深圳市疾病预防控制中心,广东深圳518055

出　　处：《实用预防医学》2015年第6期665-668,共4页Practical Preventive Medicine

基　　金：广东省医学科研基金立项项目(B2012325);深圳市科技局立项项目(201102103)

摘　　要：目的应用3种金葡菌基因分型方法分析食物中毒来源金葡菌,综合评价3种方法在金葡菌监测或暴发调查的可行性。方法应用多位点熔解曲线体系(MLMC)、葡萄球菌蛋白A序列分析(Spa typing)及脉冲场凝胶电泳(PFGE)三种基因分型技术对59株金葡菌进行分子分型。结果多位点熔解曲线分型体系将59株金葡菌分为19个型别,PFGE将其分为15个型别,Spa typing分为10个型别。Simposn差异指数分别是0.92、0.87和0.85。结论 3种分型方法应用于12起食物中毒调查,均能在同一起疫情中分析到型别一致的菌株,证实了菌株之间的亲缘关系。MLMC简单、快速,分辨率高于PFGE和Spa typing分型技术,适用于同一批暴发菌株的初筛。合理使用熔解曲线、Spa typing及PFGE分型方法对致病性金葡菌的防治具有重要意义。Objective To analyze the genotypes of Staphylococcus aureus strains isolated from the suspected food- borne dis- eases using 3 different genotyping methods, and to comprehensively evaluate the feasibility of the 3 genotyping methods for moni- toring Staphylococcus aureus and survey of the outbreak. Methods Fifty - nine strains of Staphylococcus aureus were ana- lyzed on the basis of multiple locus melting curve system （MLMC）, Staphylococcus aureus protein A （Spa） typing and pulsed field gel electrophoresis （PFGE）. Results Fifty- nine strains were classified into 19 genotypes by MLMC, 15 genotypes by PFGE and 10 genotypes by Spa typing. The Simpson＇ s diversity indices for MLMC, PFGE and Spa typing were 0.92, 0.87 and 0.85 respectively. Conclusions Twelve food poisoning outbreaks detected by the 3 genotyping methods indicate that the strains with the same genotype are found in the same outbreaks, demonstrating the genetic association of the strains. The dis- criminatory power of MLMC is higher than that of PFGE and Spa typing, and as a simple and fast genotyping method, it could be used as a first - line typing tool for analyzing outbreaks of Staphylococcus aureus. Rational combination of these 3 tools will be of great significance in pathogenic Staphylococcus aureus control and prevention.

关 键 词：金黄色葡萄球菌 基因分型 多位点熔解曲线体系 葡萄球菌蛋白A序列分析 脉冲场凝胶电泳 

分 类 号：R378[医药卫生—病原生物学]