水热法合成CdS纳米粒子及其与牛血清蛋白的共轭作用  

Hydrothermal Synthesis of CdS Nanoparticles and Its Bioconjugates with Bovine Serum Albumin

作　　者：常文贵 胡志超[1,2] 查子燕 

机构地区：[1]安徽仿生传感与检测技术省级实验室,安徽六安237012 [2]皖西学院材料与化工学院,安徽六安237012

出　　处：《皖西学院学报》2015年第2期67-71,共5页Journal of West Anhui University

基　　金：国家自然科学基金资助项目(21271141)

摘　　要：用双子表面活性剂Py-16-10-16为软模板水热法合成了CdS纳米粒子,制备的CdS纳米粒子具有较好的荧光性质。研究了CdS纳米粒子与牛血清蛋白(BSA)的共轭作用,在pH=7.40的PBS缓冲溶液中,BSA的荧光强度随着CdS纳米粒子的加入而猝灭,同时用CD光谱对BSA与CdS纳米粒子共轭作用进行了分析,这一性质说明纳米粒子与蛋白质的共轭作用,可以用于蛋白质的分析检测。In this paper, CdS nanoparticles （NPs） was hydrothermal synthesized by using Gemini surfactant, 1,10-bis（a-hexadecyl pyridine） decamethyiene dibromide （abbreviated Py-16-10-16）, as a soft template. Also, the experimental results indicated that the as synthesized CdS NPs have highly luminescent. Furthermore, when the CdS NPs were TGA-capped and then conjugated with bovine serum albumin （BSA） in PBS buffer solution （pH= 7. 40）, the fluorescence intensity of the BSA decreased with increasing contents of CdS/TGA NPs due to a static fluorescence quenching mechanism confirmed by using circular dichroism （CD） measurement. Therefore, the quenching effect of BSA on the fluorescence of TGA capped CdS NPs may find the potential applications in bioanalysis.

关 键 词：CDS纳米粒子 双子表面活性剂 牛血清蛋白 荧光猝灭 生物共轭作用 

分 类 号：TQ244.3[化学工程—有机化工]