Ag/聚L-甲硫氨酸复合修饰电极的制备及其用于果蔬中维生素C的测定  

Preparation of Ag/Poly-L-methionine Composite Modified Electrode and Determination of Vitamin C in Fruits and Vegetables

作　　者：胡文娜[1] 郑丹丹[1] 

机构地区：[1]蚌埠学院应用化学与环境工程系,安徽蚌埠233030

出　　处：《皖西学院学报》2015年第2期72-75,共4页Journal of West Anhui University

基　　金：蚌埠学院自然科学研究项目(2011ZR07)

摘　　要：采用循环伏安法将Ag和L-甲硫氨酸（L-Met）聚合在玻碳电极表面,制得Ag/聚L-甲硫氨酸复合修饰电极（AgPLM/GCE）,并对维生素C在此电极上的电化学行为进行研究,建立测定维生素C的新方法。在pH 3.0的磷酸盐缓冲溶液中,扫描速率为220mV/s时,维生素C在修饰电极上,产生一灵敏的氧化峰,峰电位为0.326V（s Ag/AgCl）,峰电流与维生素C的浓度在2.00×10-4~3.00×10-2 mol/L范围内呈良好的线性关系,检出限为5.00×10-6 mol/L。用于部分水果蔬菜中维生素C的测定,结果满意。In this paper, the silver and L-methionine were polymerized on the surface of glassy carbon electrode to prepare Ag PLM/CA2E by using CV. Focusing on the study of the electrochemical behavior of vitamin C （VC） on the modified electrode, a new method for the determination of VC was established. In the pH3.0 phosphate buffer and 220 mV/s scan rate, VC produced a sensitive oxidation peak which potential value was 0.326 V （us Ag/AgC1） in the electrode. The peak current and the concentration had a good linear relationship in the range of 2.00 × 10^-4--3.00 × 10^-2 mol/L, the detection limit was 5.00 × 10^-6 mol/L. The method was used for the determination of vitamin C in some fruits and vegetables, with the satisfactory results.

关 键 词：L-甲硫氨酸 维生素C 银 修饰电极 循环伏安法 

分 类 号：Q503[生物学—生物化学]