铜胁迫对双穗雀稗生理特性及铜积累的影响  

Effects of Cu^(2+) Stress on Physiological Properties of Paspalum Distichum and Copper Accumulation

作　　者：张静[1] 梁克中[1] 陈秀红[1] 余顺慧[1] 祁俊生[1] 

机构地区：[1]重庆三峡学院三峡库区水环境演变与污染防治重庆高校市级重点实验室,重庆万州404100

出　　处：《重庆三峡学院学报》2015年第3期109-113,共5页Journal of Chongqing Three Gorges University

基　　金：重庆高校市级重点实验室开放基金项目"三峡库区水环境演变与污染防治"(WEPKL2012MS-01)阶段性成果

摘　　要：本研究探索铜胁迫对双穗雀稗(Paspalum Distichum L.)的毒害机制,为铜污染水体植物修复提供理论依据.阐明了三峡库区消落带适生植物双穗雀稗在不同浓度Cu2+作用下新芽数、叶绿素含量、抗氧化酶系统超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及重金属铜累积与分布的影响.结果表明:在0~40 mg/L Cu2+浓度处理范围内,Cu2+浓度在0~10 mg/L时对双穗雀稗抗氧化酶系统和叶绿素含量及新芽数均有促进作用;但随着Cu2+浓度的不断增大,双穗雀稗抗氧化酶系统活性明显下降,Cu2+浓度增加到一定程度后,POD活性又稍微上升.当Cu2+浓度>10 mg/L时,双穗雀稗的新芽数和叶绿素含量减少.双穗雀稗主要通过根、茎积累Cu2+,且表现为积累量根>茎;双穗雀稗可以有效降低水体、土壤中Cu2+的含量,在三峡库区消落带库岸植物修复中具有潜在应用价值.This paper clarifies the poisoning mechanism of the copper stress on Paspalum distichum （Paspalum Distichum L.） to provide a theoretical basis for copper phytoremediation of polluted water body. In this hydroponic experiment, CuSO4.5H2O was added into Hoagland solution to simulate the Three Gorges Reservoir water pollution Cu2 ＋. It clarifies the appropriate plants in Three Gorges Reservoir with a double spike paspalum at different concentrations, Cu2 ~ effect sprout number, chlorophyll content, antioxidant enzyme superoxide dismutase system （SOD）, peroxidase （POD）, catalyse （CAT） activity and the cumulative effects and distribution of heavy metals copper. The results show that： in the 0 - 40mg L Cu2＋ inside / concentrations range, Cu2 ＋ concentration in the 0 - 10 mg / L when Paspalum distichum chlorophyll content and antioxidant enzyme systems and the number of new shoots are promoted; but with Cu2 ＋ concentration increases, Paspalum distichum decreases antioxidant enzyme system activity, Cu2 ＋ concentration increases to a certain extent, POD activity also increases slightly. When Cu2 ＋ concentration is 〉 10 mg / L, the number of shoots and chlorophyll content Paspalum distichum reduces. Paspalum distichum accumulates Cu2 ＋ mainly through the roots, stems, and the performance of the accumulation of roots is 〉 stems; Paspalum distichum can effectively reduce the water level, Cu2＋ content in soil. It is of much potential value in Phytore remedy in the Three Gorges Reservoir of Yangtze River.

关 键 词：铜胁迫 双穗雀稗 生理特性 铜积累 

分 类 号：Q945.7[生物学—植物学]