鸭甲肝病毒GX株全基因组序列测定及VP1蛋白生物信息学分析  

Determination of the Complete Genome of DHAV GX Strain and Bioinformatics Analysis of VP1 Protein

作　　者：黄云秀[1,2] 李传峰[2] 孟春春[2] 陈宗艳[2] 李露[2] 宋凯杰[2,3] 刘光清[2] 韦天超[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005 [2]中国农业科学院上海兽医研究所,上海200241 [3]西北农林科技大学动物医学院,杨凌712100

出　　处：《中国畜牧兽医》2015年第5期1039-1047,共9页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31270194;31101848;31300141);"863"计划(2011AA10A200);广西大学科研基金资助项目(XB2100113)

摘　　要：为探讨鸭甲肝病毒(DHAV)GX株基因分型特点及主要衣壳蛋白(VP1)的生物学特性,本试验对其进行全基因组序列测定,并应用分子生物学软件将DHAV GX株与DHAV 3个血清型参考毒株进行序列比对分析。结果显示,其基因组全长7 800bp,由5′和3′非编码区(UTR)和一个大开放阅读框(ORF)组成。其中,5′UTR和3′UTR的长度分别为652和369bp;ORF长度为6 756bp,编码2 251个氨基酸长的多聚蛋白,其编码产物至少有12个(VP0/VP3/VP1/2A1/2A2/2A3/2B/2C/3A/3B/3C/3D);在分类地位上DHAV GX株属于DHAV-3,其与DHAV-3参考株核苷酸、氨基酸同源性最高;与DHAV-3FS株亲缘关系最近,在同一较小分支上。DHAV GX株结构蛋白VP1以第195—201、211—221位氨基酸区段为B细胞优势表位的可能性较大。提示,VP1基因可作为研制DHAV基因工程疫苗的优势候选基因。In order to investigate the genotypic characteristics and biological characteristics of ma- jor capsid protein （VP1） of duck hepatitis A virus （DHAV） GX strain,the whole genome of GX strain was sequenced and compared with 3 serotypes reference strains of DHAV by the molecular biology software. The results showed that the full-length genome of DHAV GX strain was 7 800 bp,including 5＇UTR （652 bp）,3＇UTR （369 bp） and ORF （6 756 bp） encoding 2 251 aa and its coding products were 12 （VPO/VP3/VP1/2A1/2A2/2A3/2B/ 2C/3A/3B/3C/3D） at least. Se- quence analysis showed that DHAV GX strain could be ranked DHAV 3 style. This strain shared the highest homology with DHAV-3 reference strains; Furthermore,GX strain and DHAV-3 FS strain were in the same cluster,might come from the same ancestor. Amino acid segments locating in 195 to 201 and 211 to 221 aa could be larvaceous dominant B-cell linear epitopes. It suggested that VP1 gene could be used as a candidate gene for the development of genetic engineeringvaccine of DHAV.

关 键 词：鸭甲肝病毒 全基因组 序列分析 VP1蛋白 B细胞抗原表位 

分 类 号：S852.659.6[农业科学—基础兽医学]