蒙古绵羊Brachyury基因的克隆与生物信息学分析  

Cloning and Sequence Analysis of Mongolian Sheep Brachyury Gene

作　　者：任丽欣 智达夫[1] 李琦[1] 曹贵方[1] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010000

出　　处：《中国畜牧兽医》2015年第5期1056-1062,共7页China Animal Husbandry & Veterinary Medicine

基　　金：内蒙古农业大学科技创新项目(NDPYTD2010-6)

摘　　要：为了对蒙古绵羊Brachyury基因DNA序列进行研究,试验主要利用PCR技术对蒙古绵羊Brachyury基因序列进行扩增并克隆,首次获得了完整的Brachyury基因序列。利用NCBI网站上的BLAST功能将测序结果同已发表绵羊Brachyury基因序列进行比对,结果显示同源性高达99%,大部分序列完全一致。将该序列与GenBank数据库中登录的牛、人、大鼠、小鼠、山羊、猴和蝾螈等10个不同物种的Brachyury分子序列进行了比对分析。结果显示,蒙古绵羊Brachyury氨基酸序列与牛Brachyury氨基酸序列同源性最高,为96.30%;最低的则是来源于蝾螈的Brachyury氨基酸序列,仅为58.45%。遗传进化分析进一步证实蒙古绵羊与牛的Brachyury亲缘关系较近,与其他物种则遗传距离较远。此外,序列分析发现蒙古绵羊Brachyury基因高度保守,仅个别碱基发生突变。研究结果表明,蒙古绵羊Brachyury基因序列与其他脊椎动物的Brachyury基因序列也具有高度的保守性。In order to study Mongolian sheep Brachyury gene DNA sequence,main purpose of this article by using the method of PCR, DNA sequence of Mongolian sheep Brachyury gene cloning and the complete Brachyury gene sequences were obtained for the first time. The sequencing with NCBI BLAST function on the site, comparing the results with the published sequences of sheep Brachyury homology was as high as 99%. We compared these sequences with ten Brachyury genes of human,mouse and other animals in GenBank database. The results showed that the most sequences were identical. Mongolian sheep Brachyury shared a 96.30% amino acid homology with Bos taurus ,the highest degree of homology indicated a close genetic relationship,and the Caudata had the lowest homology 58.45 %. It could be further confirmed by phylogenetic analysis. Moreo- ver,the results showed that Mongolian sheep Brachyury gene sequence of amino acids and other vertebrates Brachyury gene sequence of amino acids were highly conservative. The research results showed that Mongolian sheep Brachyury gene sequences and other vertebrates Brachyury gene sequences were highly conservative.

关 键 词：Brachyury基因 脊椎动物 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]