反季节生产技术对鹅FSHβ和FSHR基因表达的影响  

Expression of FSHβ and FSHR Genes Respond to Goose Treated by Counter-season Production Technology

作　　者：王健[1] 张宜辉[2] 张蕊[2] 龚道清[2] 任善茂[1] 王利刚[1] 

机构地区：[1]江苏农牧科技职业学院,泰州225300 [2]扬州大学动物科学与技术学院,扬州225009

出　　处：《中国畜牧兽医》2015年第5期1063-1068,共6页China Animal Husbandry & Veterinary Medicine

基　　金：江苏省农业科技支撑项目(BE2012470);江苏省高校成果产业化项目(JHB2011-80);江苏农牧科技职业学院科研基金(PT1207)

摘　　要：为研究反季节生产技术对鹅FSHβ和FSHR基因表达的影响,选取经反季节生产技术控制的处于产蛋期和正常处于休产期扬州鹅,测定血清中FSH含量,并应用实时荧光定量PCR技术研究FSHβ和FSHR基因mRNA在下丘脑-垂体-性腺轴中的表达水平变化。结果表明,试验组(反季节生产技术控制处于产蛋期)鹅的血清中FSH水平极显著高于对照组(未调控处于休产期)鹅的血清中FSH水平(P<0.01);FSHβ和FSHR基因在垂体、下丘脑和卵巢组织中均有表达,且FSHβ在垂体中表达最高(P<0.01),FSHR基因在卵巢组织中表达最高(P<0.01);FSHβ基因在试验组鹅垂体中的表达量极显著高于其在对照组鹅垂体中的表达量(P<0.01);FSHR基因在产蛋期鹅卵巢组织中的表达极显著高于休产期鹅卵巢组织中的表达量(P<0.01)。反季节生产技术控制下鹅生殖轴组织中FSHβ和FSHR基因表达显著增加,血清中FSH测定结果与之一致,表明FSH与生殖周期密切相关。In order to study the differences of expression of FSHβ and FSHR genes respond to goose treated by counter-season production. The FSH levels in serum were detected. The pituita- ry, hypothalamus and ovarian was harvested from geese of experimental group and control group. The differences of FSHβ and FSHR genes expression in hypothalamus-pituitary-gonadal axis were detected by Real-time PCR. The results showed the FSH level of geese from experimental group was extremely significant higher than that from control group （P 〈 0. 01）. FSHβ and FSHR genes were expressed in all the three tissues. The highest expression of FSHβ was in pitui- tary while the FSHR gene was in ovarian （P〈0.01）. In the different breeding cycle,the FSHβ mRNA expression in pituitary of experimental group was extremely significant higher than that in control group （P〈0. 01）. The FSHR mRNA expression in ovarian was extremely significant higher than that in control group （P〈0.01）. Counter-season production treatment increased the expression of FSHβI gene in pituitary and FSHR gene in ovarian. The results indicated that FSH was highly associated with incubation maintenance.

关 键 词：反季节鹅 FSHΒ基因 FSHR基因 表达 

分 类 号：Q786[生物学—分子生物学]