犊牛组织中小肽转运体(POTs)的表达研究  

Expression of Proton-dependent Oligopeptide Transporters(POTs)in Calves' Tissues

作　　者：郭静[1] 杨宏波[1] 陈银岳 刘红[1] 赵国琦[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009

出　　处：《中国畜牧兽医》2015年第5期1180-1186,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家"十二五"科技支撑计划项目(2011BAD17B02);国家科技支撑项目(2012BAD34B04);江苏省科技支撑项目(BE2013387)

摘　　要：为研究犊牛部分组织中小肽转运体(proton-dependent oligopeptide transporters,POTs)(PepT1、PepT2、PHT1、PHT2)mRNA的表达,试验选用4头3月龄的中国荷斯坦犊牛进行屠宰,采用实时荧光定量PCR方法来定量组织中小肽转运蛋白表达水平。结果表明,犊牛PepT1 mRNA在瘤胃中表达丰度极显著高于心脏和肌肉(P<0.01);犊牛PepT2mRNA在肝脏和肾脏中表达丰度极显著高于心脏、脾脏、胸腺、肌肉、瘤胃、网胃、瓣胃、皱胃(P<0.01);犊牛PHT1mRNA在肺脏和脾脏中表达丰度极显著高于心脏和肌肉(P<0.01);犊牛PHT2mRNA在肺脏和胸腺中表达丰度显著高于心脏、肝脏、肾脏、脾脏、肌肉、瘤胃、网胃、瓣胃、皱胃(P<0.05)。综上所述,PepT1、PepT2、PHT1、PHT2mRNA分别在犊牛瘤胃、肾脏和肝脏、脾脏和肺脏、肺脏和胸腺中表达量最高。This test was designed to study the expression of POTs （PepT1,PepT2, PHT1 and PHT2） mRNA in calves＇tissues. Tissue-specific expression of the mRNA corresponding to pep- tide transporter protein in four 3-month-old Chinese Holstein calves was examined by relative quantitative RT-PCR analysis. The results showed that the expression of PepT1 mRNA in the ru- men was extremely significantly higher than that in the heart and muscle （P^0.01） ;The expres- sion of PepT2 mRNA in the liver and kidney was extremely significantly higher than that in the heart,spleen,thymus,muscle,rumen,reticulum,omasum and abomasum （P〈0.01） ; The expres- sion of PHT1 mRNA in the lung and spleen was extremely significantly higher than that in the heart and muscle （P〈0.01） ;The expression of PHT2 mRNA in the lung and thymus was signifi- cantly higher than that in the heart,liver,kidney, spleen,muscle,rumen,reticulum,omasum and abomasum （P〈0.05）. The results indicated that PepTI,PepT2,PHT1 and PHT2 mRNA were respectively abundantly expressed in rumen,kidney and liver, spleen and lung,lung and thymus.

关 键 词：PepT1 PepT2 PHT1 PHT2 犊牛 

分 类 号：S823[农业科学—畜牧学]