鸡呼吸道致病菌的分离鉴定  

Isolation and Identification of Respiratory Pathogens of Chicken

作　　者：孙明杰[1] 陈雷[1] 林显华[1] 崔斌[1] 王军[1] 谷巍[1] 张建梅[1] 

机构地区：[1]山东宝来利来生物工程股份有限公司,泰安271000

出　　处：《中国畜牧兽医》2015年第5期1301-1305,共5页China Animal Husbandry & Veterinary Medicine

基　　金：农用微生物制剂与酶产品创制(2013AA102800)

摘　　要：本试验旨在为开发防制鸡细菌性呼吸道疾病产品提供依据。本试验将患有呼吸道疾病的鸡病料如喉管、气管、肺脏组织接种到各种选择培养基分离细菌,对分离的17株菌株进行生化鉴定、PCR鉴定和致病力测定。结果显示,在伊红美蓝琼脂(EMB)培养基中分离出黑色金属光泽菌落;革兰氏染色后在光学显微镜下见到革兰氏阴性的短杆状细菌;PCR扩增出1 500bp大小条带,测序结果与GenBank中细菌16SrDNA序列进行BLAST比对并构建系统进化树,分离菌最终鉴定为一株大肠杆菌。将分离的致病菌滴鼻感染肉鸡能损伤肉鸡呼吸道,使喉管出现血斑,气管红肿、布满血点,心脏、肝脏包裹黄色干酪样物质,气囊浑浊不透明,具有致病性。结果表明,分离的致病菌可作为产品开发的基础。This study was aimed to provide the basis for prevention and control of development of a new product for bacterial respiratory disease of chicken. Diseased samples such as throat,trachea and lungs were inoculated on various selected media for isolating bacteria. 17 isolated strains were identified by biochemistry assay and PCR,and then animal experiment were carried out. Black and glassy surface with metallic shine colony from EMB medium Gram-negative bacilli were observed under optical microscope. 1 500 bp products were specifically amplified by PCR. DNA sequencing results were analyzed by BLAST and DNAStar with 16S rDNA sequence in GenBank. The results showed that the strains were identified as Escherichia coll. It could cause damage infection with the isolated bacteria through noses. Significant blood spots were observed on throat. Trachea was congestion,swelling and studded with blood points. Heart and liver were wrapped with yellow cheese substances. Air sac was yellow and opaque. The isolated bacteria could provide basis for developing a new product.

关 键 词：鸡 呼吸道 分离 鉴定 

分 类 号：S852.61[农业科学—基础兽医学]