不同大豆基因型对农杆菌敏感性研究及优异种质筛选  

Assessment of Agrobacterium Sensitivity for Different Soybean Genotypes and Suitable Germplasm Screening

作　　者：郭兵福[1,2] 马岩松[1,2,3] 张丽娟[2] 洪慧龙[1,2] 常汝镇[2] 郭勇[2] 邱丽娟[2] 

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京100081 [3]黑龙江省农业科学院大豆研究所,哈尔滨150086

出　　处：《植物遗传资源学报》2015年第3期569-575,共7页Journal of Plant Genetic Resources

基　　金：抗除草剂转基因大豆新品种培育重大专项(2014ZX08004001)

摘　　要：大豆遗传转化一直是植物基因工程领域的难点之一,受体品种对农杆菌的敏感程度以及不同农杆菌菌株对靶组织的侵染能力是影响转化效率的主要因素。本研究利用GUS组织瞬时表达技术,比较了4个农杆菌菌株对靶组织子叶节的侵染能力差异,同时利用筛选到的侵染能力最强的菌株评估了33个不同受体基因型对农杆菌的敏感性。结果表明,超毒农杆菌菌株Ag10对靶组织的侵染能力最强,优于其他3个菌株;地方与野生大豆种质在农杆菌侵染后GUS的平均瞬时表达效率显著高于选育品种。此外,通过鉴定筛选出6个对农杆菌敏感性较高的受体材料,其对农杆菌的敏感性优于前人报道的敏感材料Peking,为大豆高效遗传转化体系的建立和新型转化受体的开发提供了参考。Soybean is a widely planted transgenic crop all over the world. However, it is still one of the most recalcitrant crops for genetic transformation due to the genotype dependence and the hard infection. In this study, the GUS transient expression technology was used for evaluating infection ability of four Agrobacterium strains and the Agrobacterium sensitivity of 33 different soybean genotypes. The highest transient expression efficiencies of two soybean genotypes were all acquired when the explants were infected by Agrobacterium strain Ag10. Meanwhile, Agrobacterium sensitivities of 33 soybean accessions including modern cuhivars, landrace and wild soybeans were assessed and six sensitive accessions were identified when compared with a sensitive control Peking. The results also revealed that the Agrobacteriura sensitivities of landrace or wild soybeans were significant higher than that of modern cuhivars. These findings are important to construct or improve the high - efficiency genetic transformation system and develop new receptors for high efficiency genetic transformation in soybean.

关 键 词：大豆 农杆菌 敏感性 GUS染色 

分 类 号：S565.1[农业科学—作物学]