云南植物药萝芙木中51种农药残留的测定  

Determination of 51 kinds of pesticide residues in Yunnan rauvolfia

作　　者：李家雄[1] 范伢 李桂镇[1] 熊佳梁[1] 王红斌[1] 袁燕[1] 

机构地区：[1]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2015年第3期194-198,共5页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：云南民族大学绿色化学与功能材料研究省创新团队项目(2011HC008);云南省高校绿色化学新能源与材料科技创新团队项目(2011UY09);2012年国家级大学生创新创业训练计划项目(201210691001);民族药资源化学国家民委-教育部重点实验室开放基金(MZY1122)

摘　　要：用超高效液相色谱(UPLC)、双柱毛细管气相色谱(GC)、高效液相色谱-二级质谱(HPLC-MS/MS)分析方法对云南萝芙木进行51种农药残留测定.各测定方法在一定范围内线性关系良好,准确度和精密度均符合农残分析要求,在超高效液相色谱和高效液相色谱串联二级质谱中仅检测出多菌灵,含量分别为0.012 mg·kg-1和0.018 mg·kg-1,未超出国家食品中农药最大残留限量标准,没有检出其它50种农药.This research used an ultra high -performance liquid chromatography （UPLC）, and double column capillary gas chromatography （ GC ）, and high - performance liquid chromatography - the secondary mass spectrom- etry（ HPLC -MS/MS）to determine 51 species pesticide residues in Yunnan rauvolfia samples. The results showed that the above - mentioned methods had the features of a good linear relationship, precision, stability and reproduc- ibility. Only carbendazim among the 51 kinds of pesticides in the samples was detected by UPLC and HPLC - MS/ MS. Its content was 0.012 mg ·kg-1 and 0.018 mg· kg-1 respectively. The content in this sample did not exceed the limits of the maximum residue standards for food in China. Other 50 kinds of pesticides were not detected.

关 键 词：农药残留 固相萃取 超高效液相色谱 气相色谱 萝芙木 

分 类 号：R284[医药卫生—中药学] S481.8[医药卫生—中医学]