阴虚证表征的免疫性肝损伤小鼠特征及一贯煎的干预作用  

The Features of Immunological Liver Injury in Mice with Yin Deficiency Syndrome and the Intervention Effects of Yiguanjian

作　　者：田梦曦 刘文兰[1] 赵青舟[1] 欧阳罗丹 高变娥 张欣[1] 车念聪[1] 

机构地区：[1]首都医科大学中医药学院,北京100069

出　　处：《中医药导报》2015年第10期9-12,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市自然科学基金(No.81273668)

摘　　要：目的:检测免疫性肝损伤小鼠的阴虚证表征,观察一贯煎对阴虚证表征的影响。方法:将80只雄性BALB/c小鼠随机分为正常对照组、模型组、阳性对照组、一贯煎组,每组20只。除正常对照组外,均建立免疫性肝损伤小鼠模型,在造模同时,一贯煎组小鼠予一贯煎浓缩液灌胃,0.4 m L/20g,1次/d,连续8周。阳性对照组给予联苯双酯灌胃,0.2 g/kg,1次/d,连续8周。正常对照组和模型组按相同方法灌服等量生理盐水。8周后,检测各组小鼠血清ALT、AST等肝功能指标;通过HE染色评价肝脏炎症活动度(G)和纤维化程度(S);检测小鼠体质量、舌面干湿度、舌温、舌和肝脏微循环血流速度、食物和饮水、大小便等阴虚证表征。结果:(1)一贯煎在改善肝功能、减轻炎症、抗肝纤维化方面作用较好(P<0.05)。(2)免疫性肝损伤小鼠具有阴虚证的表征,一贯煎能够改善阴虚证的症状(P<0.05)。结论:免疫性肝损伤小鼠具有阴虚证的特点;一贯煎能改善肝损伤小鼠的病理及阴虚证的症状。Objective： To detect the yin deficiency symptoms of immunological liver injury in mice, and to observe the effects of traditional Chinese medicine ＂Yiguanjian＂ on characterization of yin deficiency syndromes. Method： The 80 male BALB/c mice were randomly divided into 4 groups： normal group, model group, western medicine group and Yiguanjian group, 20 mice in each. Except the normal group, liver injury model induced by Concanavalin A was established. While modeling, mice in Yiguanjian group were given Yiguanjian [0.4 mL/20（g＇d）], Western medicine group received Bifendate [0.2 g/（kg＇d）, and the normal group and model group were fed with same volume of saline daily. After 8 weeks, the serum ALT and AST were tested; the HE staining were used to evaluate the degree of liver inflammation （G） and fibrosis （S）; body weight, tongue humidity, temperature of tongue, tongue and liver microcirculation blood flow velocity, food and drinking, stool and urine were all detected. Resuhs：（1 ）Yiguanjian had good effect in improving liver function, reduce inflammation, anti liver fibrosis（P〈0.05）. （2）The mice of immunological liver injury had symptoms of Yin deficiency syndrome, and Yiguanjian can improve the symptoms of yin deficiency syndrome （P〈0.05）. Conclusions： The mice of immunological liver injury had the characteristics of Yin deficiency syndrome, and Yiguanjian can not only improve the liver injury in mice, but also could improve the symptoms of yin deficiency syndrome.

关 键 词：肝损伤 阴虚证 一贯煎 小鼠 

分 类 号：R285.5[医药卫生—中药学]