红檵木高效再生体系的建立  

Establishment of high-effi cient regeneration system of Loropetalum chinense var. rubrum

作　　者：陈容[1,2,3] 郑玉娟[2] 张党权[1,2,3] 陈丽莉[2] 龚建平 周文化[2] 何含杰[2] 覃杰明 

机构地区：[1]中南林业科技大学经济林培育与保护省部共建教育部重点实验室,湖南长沙410004 [2]中南林业科技大学林业生物技术湖南省重点实验室,湖南长沙410004 [3]中南林业科技大学经济林培育与利用湖南省2011协同创新中心,湖南长沙410004 [4]湖南澧县树大园林有限公司,湖南澧县415513

出　　处：《中南林业科技大学学报》2015年第4期40-45,共6页Journal of Central South University of Forestry & Technology

基　　金：国家林业公益性行业科研专项(201204610);湖南省科技计划重点项目(2014FJ2004)

摘　　要：红檵木是极具观赏价值的常绿灌木。以红檵木的叶片和茎段为材料,通过优化愈伤组织诱导与增殖,不定芽诱导、增殖与生根的培养基与激素配比,进行红檵木高效再生体系研究。结果表明,红檵木外植体最佳消毒方案为两次升汞消毒法：0.1%Hg Cl2两次消毒,各3 min;最佳愈伤组织诱导与增殖的培养基配方分别为：MS＋6-BA 1.0 mg/L＋NAA 1.7 mg/L、MS＋6-BA 1.0 mg/L＋IBA 0.06 mg/L;首次使用Ag NO3,获得最佳不定芽诱导培养基配方：MS＋6-BA 1.5 mg/L＋NAA 0.01 mg/L＋Ag NO3 1 mg/L;不定芽增殖最佳培养基配方为：MS＋6-BA1.0 mg/L＋IBA 0.05 mg/L＋V c 5.0 mg/L;最佳不定芽生根方案为：选择2.1-3.0 cm的不定芽,保留2-4叶片,接种至1/2 MS＋IBA 4.5 mg/L培养基。从而建立了效果稳定的完整红檵木高效再生体系,为红檵木工厂化育苗及转基因体系建立奠定基础。Loropetalum chinense var. rubrum is a kind of evergreen shrub which has great ornamental value. Using stems and leaves as explant, the high-efficient regeneration system of L. chinense var. rubrum was researched by optimizing the medium type and hormone combination in induction and multiplication of callus, induction and rooting of adventitious buds. The results show that the optimal sterilizing conditions was： disinfected twice with 0.1% HgCl2 sterilization each lasted 3 minutes; the optimal mediums for callus induction and multiplication were： MS＋6-BA 1.0 mg/L＋NAA1.7 mg/L and MS ＋ 6-BA 1.0 mg/L＋ IBA 0.06 - 0.08 mg/L, respectively; By introducing AgNO3 firstly, the optimal medium for adventitious bud induction was confirmed as： MS ＋ 6-BA 1.5 mg/L＋NAA 0.01 mg/L ＋ AgNO3 1 mg/L; the optimal medium of adventitious bud multiplication was： MS ＋ 6-BA 1.0 mg/L ＋ IBA 0.05 mg/L＋ Vc 5.0 mg/L. The optimal program for rooting of adventitious bud is as follows steps： choosing 2.1-3.0 cm tall buds with 2-4 leaves and inoculating to the medium 1/2 MS ＋ IBA 4.5 mg/L. Thereby, the high-efficient regeneration system of L. chinense var. rubrum was established, and this system provide a basis for establishing the transgenic system and implementing industrialized breeding and seedling.

关 键 词：红檵木 再生体系 不定芽诱导 硝酸银 

分 类 号：S722.37[农业科学—林木遗传育种]