急性高山病发生与低氧运动习服中人血清ET-1水平及其基因多态性  

Serum ET-1 level and ET-1 Gene Polymorphism in AMS and Sports Hypoxia Acclimation

作　　者：周文婷[1,2] 胡扬[1] 徐飞[3] 

机构地区：[1]北京体育大学,北京100084 [2]哈尔滨体育学院,黑龙江哈尔滨150001 [3]浙江工业大学体育科学研究所,浙江杭州310023

出　　处：《北京体育大学学报》2015年第4期58-64,共7页Journal of Beijing Sport University

基　　金：国家自然科学基金项目(项目编号:31071032)

摘　　要：目的:研究急性高山病(AMS)发生及低氧运动习服中血清ET-1水平的变化趋势及ET-1基因多态与其水平的关系。方法:Phase 1:模拟海拔4 800 m低氧环境,49名北方汉族大学生急性暴露6 h,入舱30min后以恒定负荷蹬车20 min,常氧安静(NMⅠ)和急性低氧暴露结束(HYⅠ)时测定血清ET-1水平。Phase 2:恢复1周后,48名受试者在模拟环境下进行3周渐进式低氧训练。恢复1周,重复Phase 1的低氧暴露和运动,常氧安静(NMⅡ)和急性低氧暴露结束(HYⅡ)时测定ET-1水平。PCR-RFLP法检测受试者ET-1基因T8000C及G5665T位点基因型和等位基因频率。结果:1)低氧暴露后受试者的ET-1水平上升不显著,AMS组与非AMS组间的ET-1变化量△1(HYⅠ-NMⅠ)差异也不显著。3周低氧训练后,低氧习服与未习服组的ET-1水平在再次低氧暴露后均上升,上升幅度△4(HYⅡ-NMⅡ)均比初次暴露时(△3(HYⅠ-NMⅠ))增多,但仅低氧习服组的ET-1水平和△4上升显著。2)G5665T及T8000C位点不同基因型和等位基因携带者的AMS发生率及AMS评分变化趋势差异均不显著。低氧暴露后,G5665T/GG和T8000C/TT组的ET-1水平上升,G5665T/GT+TT和T8000C/TC+CC组的ET-1水平下降,但各组的ET-1水平变化及组间的ET-1变化量△1(HYⅠ-NMⅠ)差异均不显著。3周低氧训练后,各基因型组的ET-1水平在低氧暴露后均比NMⅡ时上升,变化幅度△4(HYⅡ-NMⅡ)均比初次暴露后(△3(HYⅠ-NMⅠ))增加,但差异均不显著。结论:1)血清ET-1水平对低氧不敏感,ET-1变化量△1不能作为AMS易感性辅助评价指标。3周低氧训练后,低氧习服与未习服组的ET-1水平在再次低氧暴露后变化程度不同,导致两组低氧习服效果迥异。2)G5665T和T8000C位点不是AMS易感及低氧习服的遗传学标记。G5665T与T8000C位点不同基因型组的ET-1水平在初次低氧暴露时变化趋势不同,在再次低氧暴露时变化趋势相同,但ET-1水平变化仅与低氧有关,与基因多态无关。Objectives： The purpose of this study was to explore the change trend of serum ET-1 level in acute mountain sickness （AMS） and sports hypoxia acclimation, and explore the correlation between ET-1＇s gene polymorphisms and its level in serum. Methods： Phase 1 ： Forty-nine male Han nationality college students were involved in a 6 h acute hypoxia exposure with 4 800m altitudeequivalent. They rode bicycle at constant work rate for 20min after 30min adaptation to the hypoxia environment. Serum ET-1 levels were analyzed at state of normoxic resting （ NMⅠ） and the end of acute hypoxic exposure （ HYⅠ）. Phase 2 ： After one week recovery, 48 volunteers participated in 3 weeks hypoxia training （IAE） and one week recovery, finally they repeated the Phase I. ET-1 levels were analyzed at NMⅠI and HYⅠI, genotypes and alleles frequencies of ET-1 at T8000C and G5665T loci were measured by PCR-RFLP. Results ： 1 ） After hypoxia exposure, ET-1 didn＇ t increase significantly, and the a 1 （ HYⅠ - NMⅠ ） between AMS and non-AMS groups didn＇ t change. After 3 weeks hypoxia training, ET-1 of the hypoxia acclimatization group （HAG） and the hypoxia unacclimatization group （HUAG） increased after the second hypoxia exposure. The ET-1 increase of A4 （ HYⅡ - NMⅡ ） was higher than that of A3 （ HYⅠ - NMⅠ ）, but it was only happened significantly in HAG. 2 ） Occurrence rate and evaluation trend of AMS in different genotype and allele carriers at G5665T and TS000C loci didn＇ t change. After the hypoxia exposure, ET-1 level increased in G5665T/GG and T8000C/TT groups but decreased in G5665T/GT ＋ TT and T8000C/TC ＋ CC groups, the ET-1 level and ET-1 △ 1 had no differences between different groups. After 3 weeks hypoxia training, ET-I levels of the different genotype carriers increased after the second hypoxia exposure, and △4 （ HY Ⅱ - NMⅡ ） was higher than △3 （ HYⅠ - NMⅠ ） without significance. Conclusions ： 1 ） Serum ET-1 is not sensitive to h

关 键 词：急性高山病 低氧运动习服 血清ET-1水平 基因多态 

分 类 号：G804.7[文化科学—运动人体科学]