诱导烟草细胞产生抗性反应的海洋真菌的筛选  

Screening of Marine Fungus for Inducing Tobacco Cells to Produce Immune Responses

作　　者：陈晓琦[1,2] 王晓[1,3] 牟艳华[3] 王楠[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [2]中国科学院大学,北京100049 [3]沈阳药科大学,辽宁沈阳110016

出　　处：《微生物学杂志》2015年第2期42-46,共5页Journal of Microbiology

基　　金：国家自然科学基金资助项目(31471814);国家863高技术研究发展计划(2011AA09070404)

摘　　要：植物细胞胞外碱化反应及氧爆发是植物免疫反应中的重要早期事件。通过这两个细胞反应特征来发现具有潜在的诱导植物抗性反应的真菌菌株。以分离自大连凌水桥附近海域潮间带繁茂膜海绵(Hymeniacidon perleve)的50株真菌为受试菌株,通过烟草植物细胞的胞外碱化反应筛选目标菌株,发现了菌株HMP-F96的粗代谢物能够显著诱导烟草细胞发生碱化反应并产生过氧化氢。通过形态学观察和分子生物学方法鉴定该菌株为布雷正青霉(Eupenicillium brefeldianum)。为进一步研究该菌株的活性代谢产物奠定了基础。Plant extracellular alkalinization responses and oxygen explosion are important early events of plant immune responses during ISR （induced systemic resistance） establishment. In this study these two cell response characteris- tics were used to discover fungal strain that possessing latent induction of plant to react its resistance. 50 fungal strains isolated from exuberant membranous sponge （Hymeniacidon perleve） at inshore sea area near Lingshuiqiao, Dalian city were taken as tested strains, the goal strain was screened through extracellular alkalinization responses of tobacco cells, and it was found that crude metabolite of HMP-F96 could significantly induce tobacco cells to response with al- kalinization and produce hydrogen peroxide. The strain was identified as Eupenicillium brefeldianum according to mor- phological observation and molecular biology methods. This study has laid a foundation for further study on active meo tabolite of the strain.

关 键 词：植物免疫 诱导抗性 氧爆发 细胞碱化反应 布雷正青霉 

分 类 号：Q939.96[生物学—微生物学]