龟裂链霉菌中zwf基因的克隆及其生物信息学分析  

Cloning and Bioinformatics Analysis of zwf Gene in Streptomyces rimosus

作　　者：逄春梅 张萍 石彦鹏 王小娟 

机构地区：[1]宁夏泰瑞制药有限公司,宁夏银川750101

出　　处：《微生物学杂志》2015年第2期59-64,共6页Journal of Microbiology

摘　　要：利用RT-PCR技术、巢式PCR技术、3'-RACE和5'-RACE技术从龟裂链霉菌K-16菌株中克隆获得zwf基因的全长c DNA序列。测序结果表明:经软件Vector NTI 11.0拼接zwf基因全长c DNA序列为1 679 bp,并带有一段很短的poly(A)尾巴,包含1 347 bp的开放读码框(ORF),编码一个含449个氨基酸残基的蛋白质。Blast2go生物信息学分析结果表明:该基因编码的酶为葡萄糖-6-磷酸脱氢酶,通过KEGG代谢途径注释明确该基因编码的酶是参与谷胱甘肽代谢和磷酸戊糖途径代谢的关键酶。The whole length of cDNA sequence of zwf gene was cloned and obtained from Streptomyces rimosus strain K-16 adopting RT-PCR, nested PCR, 3＇-RACE, and 5＇-RACE techniques. The sequencing results showed that, the whole length of zwf gene was I 679 bp through splicing with software of Vector NTI 11.0, and it bore a short tail of po- ly（A） , containing 1 347 bp of an open reading frame （ORF） , and the code contained protein with 449 amino acids. The results of Blast2go bioinformatics analysis showed that the gene encoding enzymes was glucose-6-phosphate dehy- drogenase, it was confirmed clearly through KEGG pathways note that the genes encoded enzyme to be a key enzyme participated in glutathione metabolism and pentose phosphate pathway.

关 键 词：zwf 葡萄糖-6-磷酸脱氢酶(G6PDH) 龟裂链霉菌 土霉素生物合成 Blast2go 

分 类 号：Q939.132[生物学—微生物学] Q319.5