Identification and Diagnostics of Plant-Symbiotic and Phytopathogenic Bacteria  

作　　者：Nataliya Vladimirovna Punina Mikhail Anatolievich Remnev Alexey Fedorovich Topunov 

机构地区：[1]A. N. Bach Institute of Biochemistry RAS, Moscow 119071, Russia [2]The Federal State Unitary Enterprise All-Russia Research Institute of Automatics, Moscow 127055, Russia

出　　处：《Journal of Agricultural Science and Technology(A)》2015年第1期30-39,共10页农业科学与技术（A）

摘　　要：Rapid and reliable diagnostics and identification of pathogenic and symbiotic bacteria are at the top of the agenda. In the first case, they are important to control and prevent crop damages, and thus reduce economic losses. In the second, it＇s necessary to design and monitor quality of biofertilizer to raise its effectiveness and crop capacity. Development of accurately, rapidly, technically and commercially accessible methods remains a critical problem for the bacteria with comprehensive phylogenetic structure. In this work, we investigated pathogenic Xanthomonas and Ralstonia and symbiotic Sinorhizobium. The aim of this investigation was to examine the applicability of the novel methods for phylogenetic study, identification and diagnostics of closely related species of these genera. The conventional phenotypic and genotypic （16S rRNA, gyrB） methods were applied as referents. Novel polymerase chain reaction （PCR）-based approaches, single-adapter amplified fragment length polymorphism （saAFLP） and comparative analyses of hin-region and Xcc0006-0007 sequences, were first employed for the investigations. Phenotypic tests, 16S rRNA and gyrB analysis distinguished bacteria at the genus level, but failed to identify them to the species robustly. The new methods identified bacteria at the inter-species level more precisely. This identification agreed with the accepted genera＇s classifications. The only exceptions were X. fuscans ＆ X. cirri and X. perforance ＆ X. euvesicatoria which clustered together. The further outcome of this study was achieved hin-region-based genus-specific PCR primers for the express-diagnostics of the genera. Summary, these new methods can be applied for genome-based phylogeny investigations and as convenient and accurate tools for identification and routine laboratory diagnostics of these comprehensive genera.

关 键 词：Hin-region saAFLP XANTHOMONAS RHIZOBIUM SINORHIZOBIUM Ralstonia. 

分 类 号：S858.292[农业科学—临床兽医学] S432.4[农业科学—兽医学]