高原健康人缺氧诱导因子-1α、诱导性一氧化氮合酶和一氧化氮的海拔性变化与红细胞变形能力的关系研究  

Altitude Changes of HIF-1α,iNOS and NO in Healthy Subjects and the influence on Erythrocyte Deformability

作　　者：马秀花[1] 张鑫生[1] 郗爱旗[1] 廖宝霞[1] 李国峰[1] 

机构地区：[1]青海省人民医院老年医学研究所,810007

出　　处：《高原医学杂志》2014年第4期1-4,共4页Journal of High Altitude Medicine

摘　　要：目的:观察缺氧诱导因子-1α、诱导性一氧化氮合酶、一氧化氮和红细胞滤过指数在不同海拔的变化,探讨缺氧诱导因子-1α、诱导性一氧化氮合酶和一氧化氮的海拔性变化对红细胞变形能力的影响。方法:对不同海拔高度(西宁2 260m A组、刚察县3 300m B组、甘德县4 800m C组)320例健康人检测了缺氧诱导因子-1α(HIF-1α)、诱导性一氧化氮合酶(i NOS)、一氧化氮(NO)和红细胞滤过指数(EFI)。结果:随着海拔的升高,健康人HIF-1α水平和EFI上升,而i NOS和NO随之降低;甘德组HIF-1α水平(7.65±1.36)μg/L明显高于刚察组(5.39±1.02)μg/L和西宁组(4.18±1.14)μg/L(均P<0.01),刚察组HIF-1α水平明显高于西宁组;甘德组血清i NOS和NO(5.86±1.43)μmol/L、(38.53±4.23)μmol/L明显低于刚察组(9.44±2.13)μmol/L、(47.85±3.77)μmol/L和西宁组(12.23±2.07)μmol/L、(59.23±4.01)μmol/L(均P<0.01),同时刚察组i NOS和NO水平明显低于西宁组(P<0.01);EFI随着海拔升高而增加[西宁组:(4.67±0.49)μmol/L、刚察组:(7.05±1.51)μmol/L、甘德组:(11.45±1.78)μmol/L],各组比较有明显差异(P<0.01);相关分析表明:EFI与HIF-1α水平呈正相关(P<0.01);而与i NOS和NO呈负相关。结论:HIF-1α、i NOS和NO海拔差异性的形成在红细胞变形能力异常的病理生理机制中发挥着重要作用,而随海拔高度升高机体i NOS和NO水平的生物学特性进一步降低是导致海拔差异健康人红细胞流变形异常加重的中心环节。Objective：To observe the altitude changes of hypoxia-inducible factor-1α（HIF-1α）,inducible nitric oxide synthase（i NOS） and nitric oxide（NO）,as well as to explore the influence on erythrocyte deformability（ED） in healthy subjects.Methods：HIF-1α,i NOS,NO and erythrocyte filtration index（EFI） were measured in320 healthy subjects at different altitude（2 260 m,group Xining,3 300 m,group Gangcha and 4 080 m,group Gande）.Results：The level of HIF-1α in group Gande（7.65 ± 1.36） μg / L was higher than that in group Gangcha（5.39 ± 1.02） μg /Land group Xining（4.18 ± 1.14） μg /L（P〈0.01）; the level of HIF-1αin group Gangcha was also higher than that in group Xining（P〈0.01）,serum concentration of i NOS in group Gande（5.86 ± 1.43）μmo /L was significantly lower than that in group Gangcha（9.44 ± 2.13） μmol /L and group Xining（12.23 ±2.07） μmol / L（P〈0.01）; the contents of serum i NOS in group Gangcha was lower than that in group Xining（P〈0.01）.The serum concentration of NO in group Gande（38.53 ± 4.23） μmol / L was lower than that in group Gangcha（47.85 ± 3.77） μmol / L and group Xining（59.23 ± 4.01） μmol / L（P〈0.01）,and the contents of serum NO in group Gangcha was lower than that in group Xining（P〈0.01）.EFI increased with altitudes increasing[group Xining：（4.67 ± 0.49） μmol/L,group Gangcha：（7.05 ± 1.51） μmol/L,group Gande：（11.45 ± 1.78）μmol /L],there were significant difference at the different altitude（P〈0.01）.Correlative analyses showed that EFI was negatively correlated with i NOS and NO concentrations,and positively correlated with the level of HIF-1α.Conclusion：The formation of the different concentrations of HIF-1α,i NOS and NO may play a key role in pathophysiological mechanism of abnormalities of ED in healthy subjects at different altitude,and altitude changes on ED was closely correlated with altitude difference of biological effects i NOS and NO.

关 键 词：海拔 缺氧诱导因子-1Α 诱导性一氧化氮合酶 一氧化氮红细胞变形能力 

分 类 号：R339.54[医药卫生—人体生理学]