西宁地区ITP患者Th17相关转录因子RORγt的表达及意义  

Expression and clinical significance of RORγt in patients with immune thrombocytopenic purpura

作　　者：艾国[1] 李文倩[1] 冯建明[1] 李建平[1] 孟芳[1] 王莉[1] 沈括[1] 陈绍斌[1] 韩国雄[1] 王小蕊[1] 戴昕[1] 史艳南[1] 赵强强[1] 

机构地区：[1]青海省人民医院血液科,810007

出　　处：《高原医学杂志》2014年第4期12-14,共3页Journal of High Altitude Medicine

基　　金：国家临床重点专科建设项目;2014青海省人才"小高地"项目;青海省科技厅应用基础研究课题项目:ITP患者免疫细胞亚群及细胞因子谱研究(2012-z-704)

摘　　要：目的:检测RORγt基因在西宁地区免疫性血小板减少症(ITP)外周血单个核细胞中的表达及其临床意义。方法:应用实时荧光定量PCR对30例西宁地区免疫性血小板减少症患者及20例正常对照者外周血单个核细胞中RORγt mRNA进行检测。结果:ITP组(治疗前:0.958±0.264;治疗后:0.604±0.151)RORγt基因mRNA表达水平较对照组(0.511±0.160)升高,ITP组治疗后RORγt基因mRNA表达水平下降。治疗前后ITP患者RORγt水平与外周血血小板计数存在负相关(r=-0.739,P<0.05 and r=-0.687,P<0.05)。结论:RORγt在西宁地区ITP患者外周血单个核细胞中高表达,可能参与ITP的发生发展,可能有助于ITP的早期诊断、治疗及预后。Objective：To explore the clinical significance of RORγt expression before and after immunosuppressive therapy in the peripheral blood mononuclear cells（PBMCs） of patients with immune thrombocytopenic purpura（ITP）.Method：The real-time quantitative PCR was used to detect the expression of RORγt mRNA in 30 patients with ITP and 20 healthy controls.Results：The expression of RORγt mRNA in patients with ITP（before treatment：0.958 ± 0.264; after treatment：0.604 ± 0.151） was significantly higher than that in the healthy controls（0.604 ± 0.151）.There is a negative correlation between the expression of RORγt mRNA before and after immunosuppressive therapy in PBMCs of patients with ITP and the amount of platelets in the peripheral blood of patients with ITP（r =-0.739,P〈0.05 and r =-0.687,P〈0.05）.Conclusion：There is aberrant expression of RORγt in PBMCs of patients with ITP.It may be involved in the pathogenesis of ITP.To detect the expression of RORγt mRNA may be a reliable significance for early diagnosis,treatment and prognosis of ITP.

关 键 词：RORΓT 免疫性血小板减少症 实时定量 RT-PCR 

分 类 号：R558.2[医药卫生—血液循环系统疾病]