温阳补肾方含药血清对破骨细胞凋亡率及RANK蛋白的影响  

Effects of Serum Medicated with Wenyang Bushen Formula(温阳补肾方) on Osteoclast Apoptosis Rate and RANK Protein

作　　者：邱慈鑫 陈泽荣[1] 陈晨[1] 杨树华[1] 李楠[1] 王和鸣[1] 

机构地区：[1]福建中医药大学骨伤学院,福建福州350122

出　　处：《风湿病与关节炎》2015年第5期9-12,55,共5页Rheumatism and Arthritis

基　　金：国家自然科学基金(81173283);福建省教育厅课题(JA13156)

摘　　要：目的:探讨温阳补肾方含药血清对破骨细胞凋亡率及RANK蛋白的影响。方法:体外诱导破骨前体细胞系RAW264.7向破骨细胞分化,分别用温阳补肾方低、中、高剂量含药血清和生理盐水血清干预,通过流式细胞术检测各组凋亡率,Western Blot检测各组RANK蛋白水平。结果:与空白对照组比较,温阳补肾方含药血清可诱导破骨细胞凋亡(P=0.000),降低破骨细胞RANK蛋白的表达(P=0.000)。结论:温阳补肾方含药血清可诱导破骨细胞凋亡,温阳补肾方高剂量效果最佳。Objective：To investigate the effects of serum medicated withWenyang Bushen Formula （温阳补肾方） on osteoclast apoptosis rate and RANK protein.Methods：Differentiation in vitro was made from the osteoclast precursor cells RAW264.7 to osteoclast,which was interfered respectively with low,medium and high dose ofWenyang Bushen Formula and normal saline serum.The flow cytometry was used to detect the apop-tosis rate of each group,and the Western Blot was used to detect the level of RANK protein in each group.Results：Compared with the blank control group,serum medicated withWenyang Bushen Formula could induce apoptosis of osteoclasts（P = 0.000）,and reduce the expression of osteoclast RANK protein（P = 0.000）.Conclusion：Serum medicated withWenyang Bushen Formula can induce apoptosis of osteoclasts,high dose of which is the best.

关 键 词：破骨细胞 凋亡 RANK蛋白 温阳补肾方 含药血清 激素性股骨头坏死 

分 类 号：R285.5[医药卫生—中药学]