福辛普利对压力超负荷性心肌肥厚大鼠左心室心肌细胞bcl-2及bax基因表达的影响  

THE EFFECTS OF FOSINOPRIL ON BCL-2 AND BAX GENES OF MYOCARDIAL HYPERTROPHY RAT WITH PRESSURE VERLOAD

作　　者：辛雪[1] 薛明明[1] 

机构地区：[1]内蒙古医科大学基础医学院,内蒙古呼和浩特010010

出　　处：《内蒙古医科大学学报》2014年第5期408-411,共4页Journal of Inner Mongolia Medical University

基　　金：内蒙古自然科学基金项目(2012MS1152)

摘　　要：目的:研究福辛普利(fosinopril)对压力超负荷性心肌肥厚大鼠bcl-2、bax基因表达的影响。方法:将36只Wistar大鼠随机分为A组(假手术组)、B组(腹主动脉缩窄组)、C组(腹主动脉缩窄组+福辛普利给药组)。实验中以左心室重量指数(LVW/BW)作为判断心肌肥厚的指标;心肌细胞bcl-2、bax基因表达的变化用间接免疫荧光标记法比较。心肌细胞bcl-2、bax基因表达用流式细胞仪(Flow Cytometry FCM)测试。结果:B组与A组相比,心肌细胞bcl-2基因表达下降(P<0.01),bax基因表达增多(P<0.01);C组与B组相比,bcl-2基因表达增多(P<0.01),bax基因表达下降(P<0.05)。结论:福辛普利可促进压力超负荷性心肌肥厚大鼠bcl-2基因的表达,抑制bax基因的表达,从而使心肌肥厚发生逆转。Objective：To investgate the effects of fosinopril on the bcl-2 and bax genes of myocardial hypertrophy rat with pressure overload. Methods：36 Wistar rats were randomly divided into three groups,A group （ false operation group ） , B group （ abdominal aorta constriction group ） and C group（ abdominal aorta constriction ＋fosinopril treatment group） . The ratio of left ventricular weight vs body weight（LVW/BW）was regarded as the index of myocardial hypertrophy;Cardiomyocytes bcl-2, bax gene expression changes in comparison with the indirect immunofluorescence,which was teasted by Flow Cytometry （ FCM） . Result：Comparing with A group,B group is decreased obviously in the bcl-2 gene expression （P〈0. 01）,and increased in the bax gene expression （P〈0. 01）. Comparing with B group,C group is increased obviously in the bcl-2 gene expression （P〈0. 01）,and decreased in the bax gene expression （ P〈0 . 05 ） . Conclusion：Fosinopril could increase the expression of bcl-2 gene and decrease the expression of bax gene in the hypertrophy rats with pressure verload.

关 键 词：心肌肥厚 福辛普利 BCL-2基因 BAX基因 

分 类 号：R542.2[医药卫生—心血管疾病]