大花蕙兰丛生芽的高频诱导因素研究  

Research on High-regeneration Factors on Multiple-buds of Cymbidium hybridum

作　　者：李国树[1,2] 崔国全 罗顺聪 徐成东[1,2] 

机构地区：[1]楚雄师范学院化学与生命科学学院,云南楚雄675000 [2]滇中高原生物资源开发与利用研究所,云南楚雄675000

出　　处：《楚雄师范学院学报》2015年第3期43-47,共5页Journal of Chuxiong Normal University

基　　金：由云南省大学生创新性实验国家自然科学基金(31260095);云南省高校科技创新团队支持计划项目(IRTSTYN);生物学省级重点学科和楚雄师院校级重点学科(05YJJSXK03)项目资助

摘　　要：为研究影响大花蕙兰丛生芽的高频诱导因素,以大花蕙兰无菌原球茎进行丛生芽苗的诱导、再生及生根因素正交试验研究。结果表明:在1/2 MS+活性炭1.0g/L+NAA 0.4mg/L+6-BA 0.5mg/L+蔗糖25mg/L+琼脂10g/L的培养中,诱导率高达75.1%,丛生芽苗生长健壮;在1/2 MS+琼脂8g/L+活性炭1.0g/L+6-BA 2.5 mg/L+NAA 0.5 mg/L+蔗糖18g/L的培养基中诱导的丛生芽数量最多,达5.13倍;6-BA、NAA对大花蕙兰丛生芽的诱导及增殖作用显著;原球茎是诱导大花蕙兰丛生芽的有效方式。For the study of influencing high frequency on multiple factors of Cymbidium hybridum,by asepsis protocorm used of protocorm for growing seedlings of induction,regeneration and rooting breeding research. Results show that in 1 /2 MS ＋ AC 1. 0 g / L ＋ NAA 0. 4 mg / L ＋ 6- BA 0. 5 mg / L ＋ sucrose 25 mg / L ＋ agar cultivation of 10 g / L,the induction rate is as high as 75. 1%,cluster bud seedling growth; In 1 /2 MS ＋ agar 8 g / L ＋ 1. 0 g / L ＋ 6- BA 2. 5 mg / L ＋ NAA 0. 5 mg / L ＋ sucrose 18 g /L of medium induced the most number of multiple shoot clumps,up to 5. 13 times; 6- BA and NAA on induction of proliferation was significant; The bulb is an effective way to induce large fourth multiple shoot clumps investigate.

关 键 词：大花蕙兰原球茎 丛生芽苗 正交试验 诱导率 

分 类 号：Q949.718.430.5[生物学—植物学]