软坚散结方剂对大鼠体内5-氟尿嘧啶药代动力学行为的影响  

Effect of Ruanjian Sanjie Prescription on Pharmacokinetic Behavior of 5-Fluorouracil in Rats

作　　者：李锡晶[1] 刘国婷[2] 赵秀梅[1] 姚嫱[3] 胡人杰[1] 

机构地区：[1]天津市医药科学研究所,天津300020 [2]天津市北辰医院,天津300400 [3]天津市人民医院,天津300121

出　　处：《中国实验方剂学杂志》2015年第11期89-92,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：天津市卫生局中医;中西医结合科研专项(07038)

摘　　要：目的:考察软坚散结方剂对5-氟尿嘧啶大鼠体内药动学行为的影响。方法:大鼠单独注射5-氟尿嘧啶或联合灌胃给予软坚散结方剂,利用10%高氯酸溶液提取血浆中5-氟尿嘧啶,采用HPLC测定5-氟尿嘧啶含量,流动相0.01 mol·L-1磷酸二氢钾水溶液,流速0.8 m L·min-1,检测波长265 nm。运用Win Nonlin 5.2.1软件以非房室模型法计算药动学参数。结果:在软坚散结方剂的干预下,5-氟尿嘧啶在大鼠体内血药浓度的达峰时间显著缩短,联用组和单用组分别为(0.381±0.075),(0.514±0.122)h;表观分布容积显著减少,2组分别为(5.915±1.196),(11.307±5.393)m L;2组的其他参数均未见显著性差异。结论:软坚散结方剂能促进大鼠体内5-氟尿嘧啶的吸收,抑制后者由血液向组织中分布。Objective： To analysis effect of Ruanjian Sanjie （RJ） prescription on pharmacokinetic behavior of 5-fluorouracil （5-Fu） in rats. Method ： Rats were divided into two groups, one group was given 5-Fu only and the other one was given 5-Fu and RJ prescription together. Plasma samples were separated and extracted by 10% perchloric acid solution, HPLC was employed to determine the content of 5-Fu with mobile phase of 0.01 mol·L^-1 potassium dihydrogen phosphate, a flow rate of 0. 8 mL·min^-1 and detection wavelength at 265 nm. Pharmacokinetic parameters were calculated by WinNonlin 5.2. 1 software with non-compartmental model. Result： RJ prescription could obviously reduce Tmax [ the combined group was （0. 381 ± 0. 075） h and the single group was （0. 514 ±0. 122） hi and apparent volume of distribution [ the combined group was （5. 915 ± 1. 196） mL and the single group was （11. 307 ± 5. 393） mL] of 5-Fu in rats, but no obvious changes on other parameters. Conclusion： RJ prescription can promote absorption of 5-Fu in rats by intraperitoneal injection, while inhibiting trend of 5-Fu distribution from blood to tissues.

关 键 词：软坚散结方剂 水煎剂 5-氟尿嘧啶 药代动力学 

分 类 号：R969.1[医药卫生—药理学] R289.5[医药卫生—药学]