温胆汤对精神分裂症模型鼠海马PKC,p38 MAPK及P-Cx43的影响  

Effect of Wendan Tang on PKC,p38 MAPK and P-Cx43 of Hippocampus in Rats with Schizophrenia

作　　者：魏妍妍[1] 刘丹丹[1] 戎文娟[1] 熊怀亮 朱金华[1] 万红娇[1] 

机构地区：[1]江西中医药大学基础医学院,南昌330004

出　　处：《中国实验方剂学杂志》2015年第11期103-106,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81160423);2012级江西中医药大学校级创新基金项目(JZYC12B08)

摘　　要：目的:研究温胆汤对精神分裂症模型鼠大脑蛋白激酶C(PKC),p38丝裂原活化蛋白激酶(p38 MAPK)及磷酸化连接蛋白Cx43(P-Cx43)蛋白表达的影响。方法:将72只清洁级SD大鼠随机平均分为6组:正常组(A,生理盐水20 m L·kg-1),模型组(B,生理盐水20 m L·kg-1),氯氮平组(C,20 mg·kg-1),温胆汤高剂量组(D,40 g·kg-1),温胆汤中剂量组(E,20g·kg-1),温胆汤低剂量组(F,10 g·kg-1)。A,B组ig生理盐水;C组ig氯氮平;D,E,F组分别ig温胆汤,1次/d,21 d后,B,C,D,E,F组一次性ip地卓西平马来酸盐(MK-801)0.6 mg·kg-1,建立精神分裂症模型,行为学观察3 d后,处死大鼠,取海马组织,采用蛋白免疫印迹(Western bloting)法检测海马组织中PKC,p38 MAPK及P-Cx43蛋白的含量。结果:与正常组相比,模型组大鼠出现刻板行为;海马组织PKC,p38 MAPK及P-Cx43含量明显升高(P<0.05)。与模型组比较,温胆汤各组及氯氮平组均不同程度改善了模型鼠的一般状况;明显降低海马组织PKC,p38 MAPK及P-Cx43的含量(P<0.05)。Cx43的磷酸化程度也有统计学意义(P<0.05),且模型组磷酸化水平最高。结论:温胆汤可明显降低PKC,p38 MAPK及P-Cx43蛋白的浓度,进而调节缝隙连接通讯(GJIC)的功能。Objective： To study the effects of Wendan Tang on protein kinase C （PKC）, p38 mitogen activated protein kinase （p38 MAPK） and phosphorylated connexin43 （P-Cx43） of hippocampus in rats with schizophrenia. Method： Seventy-two SD rats were randomly averaging divided six groups： normal group （A, 20 mL·kg^-1）, model group （B, 20 mL·kg^-1）, Clozapine group （C, 20 mg·kg^-1 and Wendan Tang high- dosage group （D, 40 g·kg^-1）, Wendan Tang medium-dosage group （E, 20 g·kg^-1）, Wendan Tang low- dosage group （F, 10 g ·kg^-1）. The A and B groups were given normal saline, the C group was given Clozapine, the D, E, F groups were respectively given Wendan Tang, every day, for 21 days. The B, C, D, E, F groups were ip given dizocipine maleate （MK-801） 0. 6 mg·kg^-1to establish rat schizophrenia model, and stereotyped behavior of rats were observed. After three days, the rats were sacrificed and the hippocampus tissue was tested. The expressions of PKC, p38 MAPK, P-Cx43 in hippocampus tissue was determined by Western bloting. Result： Compared with A group, rats of B group appeared stereotyped behavior, the expressions of PKC, p38 MAPK, P-Cx43 in hippocampus tissue was increased （P 〈 0. 05）. Compared with B group, stereotyped behavior was improved, Wendan Tang could significantly decrease the expression of PKC, p38 MAPK, P-Cx43 in hippocampus tissue （P 〈 0. 05）. The degree of phosphorylation of Cx43 is also statistically significant （P 〈 0. 05） and the highest level of phosphorylation in the model group. Conclusion： Wendan Tang can significantly reduce concentration of PKC, p38 MAPK and P-Cx43, thereby regulating gap junction intercellular communication （GJIC） function.

关 键 词：温胆汤 蛋白激酶C P38丝裂原活化蛋白激酶 磷酸化连接蛋白43 缝隙连接通讯 

分 类 号：R285.5[医药卫生—中药学]