农杆菌介导的玉米茎尖遗传转化及优化  

Maize Shoot Tip Agrobacterium-mediated Transformation and Optimization

作　　者：蔡莹[1] 

机构地区：[1]中国医科大学基础医学院,辽宁沈阳110122

出　　处：《辽宁大学学报（自然科学版）》2015年第2期186-189,共4页Journal of Liaoning University：Natural Sciences Edition

摘　　要：通过用农杆菌介导法,以玉米自交系B73的种子培养成的黄化苗的茎尖作为转化的受体材料.将具有玉米C4-PEPC基因的植物表达载体p TCK303-P-ZF导入玉米自交系B73,根据载体上所带有的抗性基因,以潮霉素筛选获得抗性植株,进而为玉米遗传转化的相关研究打下坚实的基础.In this study, by using Agrobacterium mediated method,in which maize inbred lines B73 seed culture receptor material into etiolated seedlings of shoot tip as the transformation. With maize C4 - PEPC gene plant expression vector pTCK303 - P - ZF into maize inbred line B73, according to the carder＇ s resistant gene, hygromycin resistant plants were screened, so build the solid foundation for the related research of of maize genetic transformation.

关 键 词：玉米 农杆菌 茎尖转化 

分 类 号：Q37[生物学—遗传学]