表面增强拉曼光谱结合乘子效应模型对血浆和药片中甲巯咪唑的定量检测  

Quantification of Methimazole in Plasma and Tablet Samples by Surface Enhanced Raman Spectroscopy in Combination with Multiplicative Effects Model

作　　者：胡敏[1] 陈增萍[1] 陈瑶[1] 石彩霞[1] 俞汝勤[1] 

机构地区：[1]化学生物传感与计量学国家重点实验室、湖南大学化学化工学院.长沙410082

出　　处：《分析化学》2015年第5期759-764,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21275046,21475039);国家重大科学仪器设备开发专项(No.2011YQ0301240102);湖南省自然科学基金(No.2015JJ2028);教育部博士点基金(No.20130161110027);以及新世纪优秀人才支持计划(No.NCET-12-0161)资助项目~~

摘　　要：将表面增强拉曼散射（Surface-enhanced raman scattering,SERS）技术与乘子效应模型结合,采用2-巯基异烟酸作为内标,以银纳米颗粒为SERS增强基底,对血浆和药片样本中甲巯咪唑的进行准确的定量分析。实验结果表明,本方法对血浆样本中甲巯咪唑的平均相对预测误差为5.1%,检出限为32 nmol/L;对药片中甲巯咪唑的定量结果与LC-MS/MS方法基本一致,其加标回收率在93.3%~110.9%之间。The surface-enhanced Raman spectroscopy （SERS） technique in combination with multiplicative effects model （MEMSERS） was applied to the quantification of methimazole in plasma and tablet samples. Experimental results showed tfiat by using g-mercapto-isonieotinic acid as internal standard and nanosilver particles as enhanced substrate, the MEMSERS model can be applied to the accurate quantitative determination of methimazole in plasma and tablet samples. The average relative predictive error for the determination of methimazole in plasma samples was 5.1%. The recovery rates of MEMSERS for methimazole in tablet samples were in the range of 93.3% -110.9% , comparable to the corresponding values of LC-MS/ MS experiments. The limit of detection was estimated to be 32 nmol/L.

关 键 词：表面增强拉曼光谱 甲巯咪唑 2-巯基异烟酸 乘子效应模型 定量分析 

分 类 号：R927.2[医药卫生—药学] O657.37[理学—分析化学] TQ460.72[理学—化学]