自发性高血压大鼠血液中SOD、MDA、NO、ET的变化及瑞舒伐他汀干预的影响  

Changes of SOD,MDA,NO,ET from blood of spontaneously hypertensive rats,andthe influence after rosuvastatin intervention

作　　者：闫洪娟[1] 谢悦陶[2] 刘光[3] 于明月[2] 边树怀[2] 罗秋华[1] 

机构地区：[1]河北省邯郸市第一医院,河北邯郸056000 [2]河北省人民医院,河北石家庄050000 [3]河北医科大学第四医院,河北石家庄050000

出　　处：《现代中西医结合杂志》2015年第16期1727-1730,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的探讨氧自由基损害和血管内皮功能失调与高血压发生的关系及不同剂量的瑞舒伐他汀对氧化应激及内皮功能的影响。方法将8只Wistar-Kyoto大鼠(WKY)作为正常对照组;32只自发性高血压大鼠(SHR)随机分为瑞舒伐他汀3.125 mg/(kg·d)组(T1组)、瑞舒伐他汀6.25 mg/(kg·d)组(T2组)、瑞舒伐他汀12.5 mg/(kg·d)组(T3组)和SHR组,每组8只。各给药组分别给予相应剂量瑞舒伐他汀加蒸馏水1 m L灌胃,SHR组及WKY组给予相同容积的蒸馏水,共喂药4周。喂药前后测量各组大鼠血压。4周后,麻醉后抽取血液,检测血液中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、内皮素(ET)水平。结果 SHR组血液SOD活力、NO含量明显低于正常对照组,T1组、T2组、T3组明显高于SHR组。SHR组血液MDA含量、ET-1的表达明显高于正常对照组,T1组、T2组、T3组明显低于SHR组。结论SHR大鼠存在着氧化应激反应的增强和血管内皮功能损害,瑞舒伐他汀可以改善内皮功能。Objective It is to explore the relationship of oxygen free radical damage and endothelial dysfunction occurs with hypertension , and to explore the effect of different doses of rosuvastatin on hypertension oxidatie stress and endothelial function.Methods Eight Wistar-Kyoto rats （WKY） were designated as normal control group （group N）, and thirty -two spontaneously hypertensive rats （ SHR） were randomly divided into four groups , which were designated as rosuvastatin 3. 125mg/（kg？ d） group （group T1）, rosuvastatin 6.25 mg/（kg？ d） group （group T2）, rosuvastatin 12.5 mg/（kg？ d） group （group T3） and SHR control group （group H）.Each group had 8 rats.Every medication group was given the respective dose of rosuvastatin＋1 mL normal saline by gavage , and the control groups were given the same volume of tap water .All the rats were treated for 4 weeks .The blood pressure was measured by means of a noninvasive tail -cuff method before and after the 4-week treatment.In 4 weeks, blood of the rats was drew after anesthesia to determined the levels of superoxide dis -mutase （SOD）, malonaldehyde （MDA）, nitric oxide （NO） and endothelin （ET）.Results Compared with group N,the ac-tivity of SOD, the level of NO from blood were significantly lower in group H ,the indexes were higher in group T 1, group T2, group T3 compared with group H .Compared with groupn , the level of MDA , ET-1 were significantly higher in group H , the indexes were lower in group T1, group T2, group T3 compared with group H.Conclusion SHR rats have enhanced oxidative stress and damaged vascular endothelialfunction , rosuvastatin can improve their endothelial function .

关 键 词：自发性高血压大鼠 内皮功能 氧化应激反应 一氧化氮 内皮素 瑞舒伐他汀 

分 类 号：R332[医药卫生—人体生理学]