体外产气法评定青贮玉米秸与豆秸组合的瘤胃发酵特性  

Assessment of rumen fermentation characteristics between corn stalk and soybean stalk by the method of in vitro gas production

作　　者：袁翠林[1] 林英庭[1] 于子洋[1] 王文丹[1] 王利华[1] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109

出　　处：《粮食与饲料工业》2015年第5期49-53,共5页Cereal & Feed Industry

基　　金：山东省现代农业产业技术体系羊产业创新团队(SDAIT-09-011-04)

摘　　要：探讨青贮玉米秸与豆秸组合对瘤胃发酵参数的影响,以确定适宜的饲料组合比例。本试验将青贮玉米秸与豆秸分别按0∶100、20∶80、40∶60、60∶40、80∶20、100∶0的比例进行组合,利用体外瘤胃发酵技术,分析不同比例组合对产气量、pH值、氨态氮、菌体蛋白和瘤胃干物质、中性洗涤纤维和酸性洗涤纤维的影响,并计算出各组合的单项组合效应值和综合组合效应值,进而筛选出两种饲料组合的适宜比例。结果表明：青贮玉米秸-豆秸不同比例组合后在产气量上差异显著（P〈0.05）,以80∶20时最大;各比例对体外瘤胃液pH值影响差异不显著（P〉0.05）;氨态氮质量浓度随青贮玉米秸比例的增加而增加,在40~50mg/dl变化;菌体蛋白质量浓度在两者比例为80∶20时最高;青贮玉米秸-豆秸不同比例组合的干物质、中性洗涤纤维和酸性洗涤纤维的瘤胃有效降解率均以80∶20时最大。在本试验条件下,以多项组合效应评定指数评定组合效应,青贮玉米秸与豆秸比例为80∶20时较为适宜,综合组合效应指数达到最大。The experiment was conducted to investigate associative effects of different rations of corn stover（CS）and soybean stalk（SS）on fermentation.CS and SS was mixed in rations of 0∶100,20∶80,40∶60,60∶40,80∶20,100∶0,respectively.The associative effects of CS and SS were assessed by the in vitro rumen fermentation technology to monitor gas production（GP）,pH value,ammonia nitrogen（NH3N）,microbial crude protein（MCP）,rumen degradability of dry matter（DM）,neutral detergent fiber（NDF）and acid detergent fiber（ADF）,single factor associative effects index（SFAEI）and multiple factors associative effects index（MFAEI）.The results showed as follows：There were significant differences for gas production（P〈0.05）between different combinations,CS-SS in ration of 80∶20had the maximum gas production.There were not significant differences for pH value（P〉0.05）between different combinations.The NH3 N of CS-SS increased with CS increasing,and changed in the range 40~50mg/dl.The CS-SS ration of highest MCP concentration was 80∶20.The CS-SS ration of highest rumen degradability of DM,NDF and ADF was 80∶20.Under the conditions of this experiment,with the combination of multiple effect as evaluation index,it was include from MFAEI that the combination of CS-SS in ration of 80∶20were appropriate,the comprehensive effect index reached the maximum.

关 键 词：体外产气法 青贮玉米秸 豆秸 组合效应 瘤胃降解率 

分 类 号：S816[农业科学—饲料科学]