中药复方艾可清加味对艾滋病模型猴不同组织CD4分子表达量的干预作用  

Intervention of traditional Chinese medicine Viagra flavored clear expre ssion of the CD4 molecule on monkeys with AIDS in different tissues

作　　者：朱伯强 符林春[1] 张苗苗[1] 

机构地区：[1]广州中医药大学热带医学研究所,广东广州510405

出　　处：《中国民族民间医药》2015年第10期7-9,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：国家自然基金项目(编号:81173436)

摘　　要：目的:以艾滋病模型猴不同组织的CD4分子表达为靶点,比较艾滋病模型猴不同组织中CD4分子的表达,探讨中药复方艾可清加味对艾滋病模型猴不同组织CD4分子表达的影响。方法:通过标准方法建立艾滋病猴模型,分别设立正常对照组、模型对照组、中药组和阳性药物组。正常组不作任何处理,模型组给予生理盐水灌胃,中药组给予中药复方煎剂灌胃,阳性药物组给予替诺福韦皮下注射治疗。治疗结束后取材相关组织,用Western blot分析模型猴不同组织的CD4分子的表达。结果:艾滋病模型猴的胃、十二指肠、回肠、结肠、直肠、肠系膜淋巴结、脾、浅表淋巴结织组中CD4表达水平中药组均数值与模型组比较分别高出11.69%、10.67%、54.55%、15.31%、27.36%、22.68%、2.11%、10.75%。结论:中药复方可以有效调节艾滋病模型猴中不同组织的免疫情况,提升CD4的表达,改善免疫功能,对猴艾滋病有一定治疗作用。Objective The monkey model of AIDS CD4 molecule expression in different tissues as a target,compare expression monkey model of AIDS CD4 molecules in different tissues to study the effects of traditional Chinese medicine monkey model of AIDS CD4 molecule expression in different tissues.Methods AIDS monkey model by standard methods,namely the establishment of the nor-mal control group,model group,TCMgroup and positive drug group.Normal group without any treatment,the model group was given normal saline,TCM group were given traditional Chinese medicine decoction,positive drug tenofovir group was given subcutaneous treatment.Minutes after the end of treatment-related organizations drawn expression monkey model of CD4 molecules in different tissues analyzed by Western blot.Results AIDS model monkey stomach,duodenum,ileum,colon,rectum,mesenteric lymph nodes,spleen, lymph node tissue superficial group TCMgroup CD4 expression levels were higher than the value of the model group were 11.69%, 10.67%,54.55%,15.31%,27.36%,22.68%,2.11%,10.75%.Conclusion The traditional Chinese medicine can effectively regulate the immune monkey model of AIDS cases in different organizations,to enhance the expression of CD4,improve immune func-tion,AIDS monkeys have some therapeutic effect.

关 键 词：艾滋病 CD4 分子 中国恒河猴 免疫印迹 中药复方 

分 类 号：R285.5[医药卫生—中药学]