冰糖草中总酚酸的含量测定  

Determination of Total Phenolic Acid in Scoparia dulcis

作　　者：陈丽珍[1] 艾朝晖[1] 蔡蓝洁[1] 李娟[1] 翟锐锐[1] 付煜荣[1] 

机构地区：[1]海南医学院药学院,海南海口571199

出　　处：《中国民族民间医药》2015年第10期10-10,12,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立冰糖草水提液中总酚酸的含量测定方法。方法:采用分光光度法,以没食子酸为对照品,0.6%Fe Cl3-0.9%K3[Fe(CN)6](1∶0.9)混合液为显色剂,在720nm波长处进行冰糖草中总酚酸的含量测定。结果:所测定总酚酸的线性相关系数为0.9995,精密度≤0.99%,平均回收率为99.4%,RSD≤2.31%,重现性好。结论:该方法简便快、准确、稳定,可作为冰糖草总酚酸含量测定的方法。Objective To establish the methods for detection of total phenolic acid in Scoparia dulcis.Methods Total phenolic acid was analyzed by spectrophotometry at 720nm.Gallic acid as a reference,the colour reaction agent was the mixture solution of 0.6%FeCl3 -0.9%K3 [Fe （CN）6 ]（1∶0.9）.Results The linear correlation coefficient of total phenolic acid was 0.9995.The precisions were less than 0.99%;the average recovery was 99.4% with RSD of less 2.31%.Conclusion The method was proved to be fast, simple,accurate and reliable for the quality control of gallic acid and total phenolic acid in Scoparia dulcis.

关 键 词：冰糖草 总酚酸 含量测定 分光光度法 

分 类 号：R284.1[医药卫生—中药学]