内生真菌和丛枝菌根真菌对羊草生长的影响  

Effects of endophyte and arbuscular mycorrhizal fungi on growth of Leymus chinensis

作　　者：刘慧[1] 陈薇[1] 周勇[1] 李夏[1] 任安芝[1] 高玉葆[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《植物生态学报》2015年第5期477-485,共9页Chinese Journal of Plant Ecology

基　　金：国家自然科学基金(31270463);教育部博士点基金项目(20130031110023)

摘　　要：该研究比较了摩西球囊霉(Glmous mosseae)和幼套球囊霉(Glmous etunicatum)两种丛枝菌根真菌和内生真菌单独及混合接种对羊草(Leymus chinensis)生长的影响。结果表明,内生真菌对2种菌根真菌的侵染均无显著影响,内生真菌可极显著增加羊草的分蘖数、地上生物量、总生物量。内生真菌与菌根真菌之间的相互作用因菌根真菌种类而不同,幼套球囊霉对宿主植物生长无明显影响且和内生真菌之间也无明显的相互作用;单独接种摩西球囊霉显著增加羊草的地上、地下和总生物量,当其与内生真菌共同存在时,二者之间存在一定的拮抗作用。冗余分析结果表明,在内生真菌-AM真菌-羊草共生体中,内生真菌对宿主植物生长的影响最大,摩西球囊霉对宿主植物生长也有一定的贡献,幼套球囊霉对宿主植物生长无明显影响。Aims Endophyte not only enhance plant resistance to biotic and abiotic stress but also have the positive effect on the growth of the host plants. Arbuscular mycorrhizal fungi （AMF） can improve plant mineral nutrition uptake and storage. Endophyte and AMF coexist in Leymus chinensis. Our objective was to investigate the effects of endophyte and/or AMF on the growth of Leymus chinensis. Methods We conducted a pot experiment combining two endophyte infection status （endophyte-infected, EI;endophyte-free, EF） and three mycorrhizal inoculation treatments （Glmous etunicatum, Ge;G. mosseae, Gm and non-inoculation, M–）, using L. chinensis plants as a model. There were five replicates per treatment. The experi-ment was carried out at the campus experimental field at Nankai University. Important findings Endophyte presence had no impact on the hyphal colonization of the two AMF species, but significantly increased tillers, shoot biomass and total biomass of L. chinensis. Interspecific symbiotic interactions varied with partner identity. Glmous etunicatum had no impacts on plant growth and no interaction with endo-phyte;G. mosseae alone significantly increased plant shoot, root and total biomass but had antagonistic impact on endophyte when coexisting with endophyte. Redundancy analysis showed that endophyte had the largest impact on the host growth, G. mosseae contributed to plant growth to some extent and G. etunicatum had no significant effect on the endophyte and negligible impacts on plant biomass.

关 键 词：内生真菌 幼套球囊霉 摩西球囊霉 互作 羊草 

分 类 号：S543.9[农业科学—作物学]