不同处理条件对库普曼卫矛愈伤组织诱导的影响  

Effect of Euonymus koopmannii Callus Induction in Different Treatment Conditions

作　　者：李军正[1] 梁凤丽[1] 余天蓝 苟林[1] 

机构地区：[1]新疆农业大学林学与园艺学院,新疆乌鲁木齐830052

出　　处：《园艺与种苗》2015年第4期55-57,共3页Horticulture & Seed

基　　金：干旱区生物多样性保育与恢复生态学科研创新团队资助;新疆农业大学大学生创新项目(jqztp72013172):卫矛属两种植物种子萌发及幼苗生长特性研究

摘　　要：[目的]筛选库普曼卫矛叶片愈伤组织诱导的适宜条件,为建立完整的库普曼卫矛组织培养体系奠定基础。[方法]以库普曼卫矛叶片为外植体,通过75%酒精＋84消毒液＋0.1%升汞不同时间消毒处理,寻求外植体最优消毒方法。分别采用MS、1/2MS、WPM 3种类型基本培养基寻求最适宜愈伤组织生长的基本培养基类型。以MS为基本培养基,通过6-BA与IBA不同浓度激素的搭配组合,寻求最适宜愈伤组织生长的培养基类型。[结果]不同消毒处理中尤以0.1%的升汞消毒5 min效果最好;不同基本培养基中尤以MS培养基处理效果最理想;不同激素组合中外植体愈伤组织在MS＋6-BA 3.0 mg/L＋IBA 0.2 mg/L中生长最好。[结论]在p H 5.8-6.0条件下,外植体采用75%酒精0.5 min＋0.1%升汞5 min消毒处理,转入MS＋6-BA 3.0 mg/L＋IBA 0.2 mg/L＋30 g蔗糖＋7 g琼脂的培养基中所诱导出的愈伤组织生长最好,效果最显著。[Objective] The optimum condition of Euonymus koopmannii leaf inducing callus medium was selected. It would provide basis for Euonymus koopmannii tissue culture system. [Methods] The leaves were treated as the explants with different concentration combination of 75 % alcohol, 84 disinfectant and 0.1% mercuric. 3 types basic culture medium of MS, 1/2MS,WPM were used to confirm the optimal medium for the growth of callus. Through the adoption of MS as basic medium and the combination of different hormone concentration of 6-BA and IBA, the optimal medium for the growth of callus were founded. [Results] The disinfection with 0.1% mercuric for 5 minutes was proved as the best. MS medium was the best effect compared with other basic culture mediums. The hormone combination of MS＋6-BA 3.0 mg/L＋IBA 0.2 mg/l, was the best for explants callus. [Conclusion] Under pH 5.8-6.0 the best combined project of disinfection with 75% alcohol for 30 seconds and 0.1% mercuric for 5 minutes, and culture mediums with MS＋6-BA 3.0 mg/L＋ IBA 0.2 rag/L＋30 g sucrose ＋7 g agar were proved as the best for inducing callus.

关 键 词：库普曼卫矛 愈伤组织 消毒处理 基本培养基 激素组合 

分 类 号：S685[农业科学—观赏园艺]