以钯(Ⅱ)与吖啶红形成的络合物作为荧光探针荧光光谱法测定L-异亮氨酸  

Fluorescence Spectroscopy Determination of L-Isoleucine with Complex of Pd(Ⅱ)and Acridine Red as Fluorescent Probe

作　　者：程定玺[1] 朱会宇 刘起胜[1] 

机构地区：[1]河南师范大学化学化工学院,新乡453007

出　　处：《理化检验（化学分册）》2015年第5期651-654,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：在pH 10.7的Kolthoff缓冲溶液中,钯（Ⅱ）能与吖啶红形成络合物从而使其荧光发生静态淬灭。当加入L-异亮氨酸后,L-异亮氨酸能与钯（Ⅱ）形成更为稳定的络合物而释放出吖啶红,使体系的荧光强度大幅增加。据此,提出了以钯（Ⅱ）与吖啶红形成的络合物作为荧光探针的荧光光谱法测定L-异亮氨酸的方法。激发和发射光谱通带宽度分别为5,10nm,反应时间为120min。L-异亮氨酸的线性范围为0.125~1.375 mg·L-1,方法的检出限（3s/k）为0.27μg·L-1。L-异亮氨酸与钯（Ⅱ）的络合比为3比1。加标回收率在106%~114%之间,测定值的相对标准偏差（n=6）小于4.0%。In Kolthoff buffer at pH 10. 7, Pd（ Ⅱ ） and acridine red could form a complex, which quenches the fluorescence intensity of acridine red. After adding L-isoleucine, a more stable complex was formed and was free acridine red release, which significantly enhanced the fluorescence intensity of the system. Based on this fact, a fluorospectrophotometric method with fluorescence probe of complex of Pd（Ⅱ ） and acridine red was proposed for the determination of L-isoleucine. The excitation slit width was 5 nm and emission slit width was 10 nm. The reaction time was 120 min. The linearity range of L-isoleucine 0. 125-1. 375 mg·L-1 , with detection limit （3s/k） of 0. 27μg·L-1. The complexation ratio of Pd（ Ⅱ ） to L-isoleucine was 3 ：1. The recovery rates measured by standard addition method were in the range of 106%-114%, with RSD （n=6） less than 4. 0%.

关 键 词：荧光光谱法 L-异亮氨酸 吖啶红 络合物 

分 类 号：O657.31[理学—分析化学]