九香虫水提液对两种癌细胞的作用研究  

Effects of Extracts from Aspongopus chinensis Dallas on Cancer Cells

作　　者：于声[1] 段斯亮[1] 李海叶[2] 黄富平[2] 

机构地区：[1]广西科技大学医学院,广西柳州545005 [2]广西师范大学化学与药学学院,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2015年第1期104-108,共5页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金资助项目(21101035);广西自然科学基金资助项目(2012GXNSFBA053017);药用资源化学与药物分子工程教育部重点实验室资助项目(CMEMR2013-A05)

摘　　要：本文研究九香虫水提取液对人胃癌细胞SGC-7901和肝癌细胞HepG2增殖抑制及细胞周期的影响。设置九香虫水提液药物组和正常对照组,应用倒置显微镜观察细胞形态;采用MTT法观察水提液对两种细胞的增殖抑制作用;利用流式细胞术分析其对两种细胞周期的影响。结果显示,九香虫水提液可使两种细胞变圆,脱壁细胞增多,细胞间空隙增大,细胞碎片增多;MTT实验显示,水提液能抑制SGC-7901细胞和HepG2细胞的体外增殖且呈明显的剂量依赖性,对SGC-7901细胞和HepG2细胞的IC50分别为154.24、129.46mg/L。流式细胞术结果显示,九香虫水提液使两种细胞的S期和G2/M期比例增加,G0/G1期细胞比例降低,表明九香虫水提取液能抑制SGC-7901和HepG2细胞的体外增殖,其机制可能是由于细胞周期的阻滞。To study the effects of water extract of Aspongopus chinensis Dallas on cell proliferation and cell cycle of SGC-7901 and HepG2 cell lines, by using inverse microscopy, the morphology of the cells were observed. The antiproliferative effects of water extracts were assessed by MTT assays. The cell cycle was measured by flow cytometry. The results showed that after being treated with water extracts, cells grew round ,and the intercellular space were increased, cell fragmentation were increased and cells were against the wall in the inverse microscopy. The water extracts could inhibit the proliferation of SGC-7901 and HepG2 cell lines in a dose-dependent manner. SGC-7901 and HepG2 cell lines were sensitive to water extract with an ICs0 values of 154.24 mg/L and 129.46 mg/L, respectively. From the result of flow cytometry analysis,water extract, as compared with the untreated cells, caused significant increase in cell ratio at the S and G2/M phases, and anreduction in cell ratio at the G0/G1 phases. The water extract could inhibit proliferation of both cancer cell in vitro, and especially arrest cancer cells at the S phase of the cell cycle for SGC-7901 and HepG2 cell.

关 键 词：九香虫 水提取液 细胞增殖 细胞周期 SGC-7901 HEPG2 

分 类 号：R285.5[医药卫生—中药学] R931.74[医药卫生—中医学]