意大利蜜蜂工蜂幼虫饲粮中亚油酸的适宜添加水平  

Appropriate Linoleic Acid Supplemental Level in Apis mellifera ligustica Worker Larvae Diet

作　　者：王圣伟[1] 胥保华[1] 王红芳[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《动物营养学报》2015年第5期1440-1449,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家蜂产业技术体系建设专项资金(CARS-45);山东省农业良种工程项目"优质高产蜜蜂及蚕桑新品种培育"(2014-2016)

摘　　要：本试验旨在研究意大利蜜蜂工蜂幼虫饲粮中亚油酸的适宜添加水平。选取576只1日龄的工蜂幼虫,随机分为6组,每组96只幼虫,对照饲喂基础饲粮,试验组分别饲喂添加0.02%、0.04%、0.06%、0.08%和0.10%亚油酸的饲粮,试验期7 d。结果表明：1）与对照组相比,饲粮添加0.10%亚油酸显著降低了化蛹率（P〈0.05）。2）与对照组相比,饲粮添加0.04%亚油酸显著提高了5日龄虫体蛋白质浓度（P〈0.05）;饲粮中添加0.10%亚油酸显著降低了7日龄蛹蛋白质浓度（P〈0.05）。3）与对照组相比,饲粮添加0.02%亚油酸对6日龄虫体的过氧化物酶增殖物激活受体（PPAR）α活性有显著的抑制作用（P〈0.05）,添加0.08%的亚油酸则显著提高了6日龄虫体PPARα和PPARγ活性。4）与对照组相比,饲粮添加0.04%和0.08%亚油酸显著提高了6日龄虫体脂肪酸合成酶（FAS）活性（P〈0.05）;添加0.08%亚油酸显著提高了5日龄虫体乙酰辅酶A羧化酶（ACC）浓度（P〈0.05）,添加0.06%和0.10%亚油酸显著提高了6日龄虫体ACC浓度（P〈0.05）。5）饲料中添加本试验设定范围的亚油酸对幼虫FAS和ACC基因相对表达量无显著影响（P〉0.05）。综合化蛹率和虫体蛋白质浓度2个指标的拟合曲线结果,认为意大利蜜蜂工蜂幼虫饲粮中亚油酸的适宜添加水平为0.024%~0.037%。This experiment was conducted to study the appropriate linoleic acid supplemental level in Apis mel- lifera ligustica worker larvae diet. Five hundred and seventy six larvae were randomly assigned to 6 groups with 96 larvae in each group. Larvae in control group were fed a basal diet, and those in experimental groups were fed the basal diet supplemented with 0.02%, 0.04%, 0.06%, 0.08% and 0.10% linoleic acid, respectively. The experiment lasted for 7 days. The results showed as follows ： 1 ） compared with the control group, the diet supplemented with 0.10% linoleic acid reduced the percentage of pupation （P〈0.05）. 2） Compared with the control group, the diet supplemented with 0.04% linoleic acid significantly increased the total protein concen- tration of larvae at 5 days old （P〈0.05）, when the worker larvae developed into pupa at 7 days old, the diet supplemented with 0.10% linoleic acid significantly increased the pupae protein concentration （P〈0.05）. 3 ） Compared with the control group, the diet supplemented with 0.02% linoleic acid had significant suppression effects on the activity of peroxisome proliferators-activated receptor （PPAR）et （P〈0.05）, but 0.08% linoleic acid had stimulating effects on the activities of PPARct and PPAR.y at 6 days old. 4） Compared with the control group, the linoleic acid supplemental levels at 0.04% and 0.08% significantly improved the activity of fatty acid synthetase （FAS） of larvae at 6 days old （P〈0.05）. Acetyl CoA carboxylase （ACC） concentration of larvae at 5 days old was significantly improved by 0.08% linoleic acid （P〈0.05） and ACC concentration of larvae at 6 days old was significantly improved by 0.06% and 0.10% linoleic acid （P〈0.05）. 5） Different supplemental levels of linoleic acid had no effects on the relative expression levels of FAS and ACC genes （ P〉 0.05）. According to the curve fitting results of the percentage of pupation and protein concentration of larvae, it is concluded that the appro

关 键 词：亚油酸 意大利蜜蜂 适宜添加水平 化蛹率 蛋白浓度 脂肪代谢 

分 类 号：S816[农业科学—饲料科学]